Observing in-phase single-quantum 15N multiplets for NH2 / NH3 + groups with two-dimensional heteronuclear correlation spectroscopy

Yuki Takayama, Debashish Sahu, Junji Iwahara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Two-dimensional (2D) F1-1H-coupled HSQC experiments provide 3:1:1:3 and 1:0:1 multiplets for AX3 and AX2 spin systems, respectively. These multiplets occur because, in addition to the 2 Sy Hz a → 2 Sy Hz a process, the coherence transfers such as 2 Sy Hz a → 2 Sy Hz b occurring in t1 period provide detectable magnetization during the t2 period. Here, we present a 2D F1-1H-coupled 1H-15N heteronuclear correlation experiment that provides a 1:3:3:1 quartet for AX3 spin system and a 1:2:1 triplet for AX2. The experiment is a derivative of 2D HISQC experiment [J. Iwahara, Y.S. Jung, G.M. Clore, Heteronuclear NMR spectroscopy for lysine NH3 groups in proteins: unique effect of water exchange on 15N transverse relaxation. J. Am. Chem. Soc. 129 (2007) 2971-2980] and contains a scheme that kills anti-phase single-quantum terms generated in the t1 period. The purge scheme is essential to observe in-phase single-quantum multiplets. Applications to the NH2 and NH3 + groups in proteins are demonstrated.

Original languageEnglish
Pages (from-to)313-316
Number of pages4
JournalJournal of Magnetic Resonance
Volume194
Issue number2
DOIs
StatePublished - Oct 2008

Fingerprint

Spectrum Analysis
fine structure
Spectroscopy
Biomolecular Nuclear Magnetic Resonance
spectroscopy
Lysine
proteins
Proteins
Magnetic Resonance Spectroscopy
lysine
Experiments
Water
Nuclear magnetic resonance spectroscopy
Magnetization
magnetization
nuclear magnetic resonance
Derivatives
water

Keywords

  • N
  • AX spin system
  • AX spin system
  • Heteronuclear correlation
  • Multiplets

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics

Cite this

Observing in-phase single-quantum 15N multiplets for NH2 / NH3 + groups with two-dimensional heteronuclear correlation spectroscopy. / Takayama, Yuki; Sahu, Debashish; Iwahara, Junji.

In: Journal of Magnetic Resonance, Vol. 194, No. 2, 10.2008, p. 313-316.

Research output: Contribution to journalArticle

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