Occult systemic infection and persistent simian immunodeficiency virus (SIV)-specific CD4+-T-cell proliferative responses in rhesus macaques that were transiently viremic after intravaginal inoculation of SIV

Michael B. McChesney, Jennifer R. Collins, Ding Lu, Xusheng Lu, Judith Torten, Rhoda L. Ashley, Miles W. Cloyd, Christopher J. Miller

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Abstract

The intact cervicovaginal mucosa is a relative barrier to the sexual transmission of human immunodeficieney virus type 1 (HIV-1). In the simian immunodeficiency virus (SIV) macaque model of HIV infection, seronegative transient viremia (STV; virus isolation positive followed by repeated negative cultures) occurs after intravaginal inoculation of a low dose of pathogenic SIVmac251 (C. J. Miller, M. Marthas, J. Torten, N. Alexander, J. Moore, G. Doncel, and A. Hendrickx, J. Virol. 68:6391-6400, 1994). Thirty- one adult female macaques that had been inoculated intravaginally with pathogenic SIVmac251 became transiently viremic. One monkey that had been culture negative for a year after SIV inoculation became persistently viremic and developed simian AIDS. No other STV monkey developed persistent viremia or disease. Results of very sensitive assays showed that 6 of 31 monkeys had weak SIV-specific antibody responses. SIV-specific antibodies were not detected in the cervicovaginal secretions of 10 STV monkeys examined. Twenty of 26 monkeys had lymphocyte proliferative responses to p55(gag) and/or gp130(env) antigens; 3 of 6 animals, including the monkey that became persistently viremic, had detectable cytotoxic T-lymphocyte (CTL) responses to SIV. At necropsy, lymphoid tissues and vaginal mucosa were virus culture negative, but in 10 of 10 animals, SIV provirus was detected by PCR using gag-specific primer pairs. Fifty percent of the PCR-positive tissue samples were also positive for SIV gag RNA by reverse transcriptase PCR. Thus, transient viremia following intravaginal inoculation of pathogenic SIV is associated with persistent, systemic infection, either latent or very low level productive. Atypical immune responses, characterized by lymphocyte proliferation and some CTL responses in the absence of conventionally detectable antibodies, develop in transiently viremic monkeys.

Original languageEnglish (US)
Pages (from-to)10029-10035
Number of pages7
JournalJournal of Virology
Volume72
Issue number12
StatePublished - Dec 1998

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca mulatta
Haplorhini
monkeys
T-lymphocytes
vaccination
T-Lymphocytes
Infection
infection
Viremia
viremia
cytotoxic T-lymphocytes
Macaca
Cytotoxic T-Lymphocytes
Viruses
antibodies
Simian Acquired Immunodeficiency Syndrome
Mucous Membrane
vaginal mucosa

ASJC Scopus subject areas

  • Immunology

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Occult systemic infection and persistent simian immunodeficiency virus (SIV)-specific CD4+-T-cell proliferative responses in rhesus macaques that were transiently viremic after intravaginal inoculation of SIV. / McChesney, Michael B.; Collins, Jennifer R.; Lu, Ding; Lu, Xusheng; Torten, Judith; Ashley, Rhoda L.; Cloyd, Miles W.; Miller, Christopher J.

In: Journal of Virology, Vol. 72, No. 12, 12.1998, p. 10029-10035.

Research output: Contribution to journalArticle

McChesney, Michael B. ; Collins, Jennifer R. ; Lu, Ding ; Lu, Xusheng ; Torten, Judith ; Ashley, Rhoda L. ; Cloyd, Miles W. ; Miller, Christopher J. / Occult systemic infection and persistent simian immunodeficiency virus (SIV)-specific CD4+-T-cell proliferative responses in rhesus macaques that were transiently viremic after intravaginal inoculation of SIV. In: Journal of Virology. 1998 ; Vol. 72, No. 12. pp. 10029-10035.
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abstract = "The intact cervicovaginal mucosa is a relative barrier to the sexual transmission of human immunodeficieney virus type 1 (HIV-1). In the simian immunodeficiency virus (SIV) macaque model of HIV infection, seronegative transient viremia (STV; virus isolation positive followed by repeated negative cultures) occurs after intravaginal inoculation of a low dose of pathogenic SIVmac251 (C. J. Miller, M. Marthas, J. Torten, N. Alexander, J. Moore, G. Doncel, and A. Hendrickx, J. Virol. 68:6391-6400, 1994). Thirty- one adult female macaques that had been inoculated intravaginally with pathogenic SIVmac251 became transiently viremic. One monkey that had been culture negative for a year after SIV inoculation became persistently viremic and developed simian AIDS. No other STV monkey developed persistent viremia or disease. Results of very sensitive assays showed that 6 of 31 monkeys had weak SIV-specific antibody responses. SIV-specific antibodies were not detected in the cervicovaginal secretions of 10 STV monkeys examined. Twenty of 26 monkeys had lymphocyte proliferative responses to p55(gag) and/or gp130(env) antigens; 3 of 6 animals, including the monkey that became persistently viremic, had detectable cytotoxic T-lymphocyte (CTL) responses to SIV. At necropsy, lymphoid tissues and vaginal mucosa were virus culture negative, but in 10 of 10 animals, SIV provirus was detected by PCR using gag-specific primer pairs. Fifty percent of the PCR-positive tissue samples were also positive for SIV gag RNA by reverse transcriptase PCR. Thus, transient viremia following intravaginal inoculation of pathogenic SIV is associated with persistent, systemic infection, either latent or very low level productive. Atypical immune responses, characterized by lymphocyte proliferation and some CTL responses in the absence of conventionally detectable antibodies, develop in transiently viremic monkeys.",
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AU - Collins, Jennifer R.

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