Occurrence of a furin-like prohormone processing enzyme in Aplysia neuroendocrine bag cells

Gregg T. Nagle, Walter R A van Heumen, Susan L. Knock, Anna T. Garcia, David A. McCullough, Alexander Kurosky

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    1. 1. Strong evidence is accumulating that the endoproteases which process prohormones at dibasic residue cleavage sites are members of a subtilisin-related class of proteases. 2. 2. Using the polymerase chain reaction (PCR), we have isolated and characterized an Aplysia californica neuroendocrine bag cell cDNA product (270 base pairs) that encodes a sequence which is highly homologous to the subtilisin-related class of processing proteases that includes yeast Kex2, human/mouse/Drosophila furin, human PC2, and mouse PC1/PC3 and PC2. 3. 3. The characterized cDNA PCR product showed the highest degree of residue identity with the furin-related group of proteins (human/mouse furin 71%; Drosophila furin 63%). 4. 4. These results establish that Aplysia contain a subtilisin-like gene and suggest that the expression of this gene may play a role in processing Aplysia precursor proteins in the bag cells and likely also in the exocrine atrial gland. 5. 5. Furthermore, the Aplysia nucleotide sequence results, together with available sequence information from human, mouse, and Drosophila furins, provide reasonable evidence that the furin-like enzymes may represent a separate subclass of the subtilisin-like processing enzymes.

    Original languageEnglish (US)
    Pages (from-to)345-348
    Number of pages4
    JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
    Volume105
    Issue number2
    DOIs
    StatePublished - 1993

    Fingerprint

    Furin
    Aplysia
    Neuroendocrine Cells
    Subtilisin
    Enzymes
    Processing
    Drosophila
    Polymerase chain reaction
    Peptide Hydrolases
    Complementary DNA
    Genes
    Exocrine Glands
    Polymerase Chain Reaction
    Protein Precursors
    Reaction products
    Base Pairing
    Yeast
    Nucleotides
    Yeasts
    Gene Expression

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology

    Cite this

    Occurrence of a furin-like prohormone processing enzyme in Aplysia neuroendocrine bag cells. / Nagle, Gregg T.; van Heumen, Walter R A; Knock, Susan L.; Garcia, Anna T.; McCullough, David A.; Kurosky, Alexander.

    In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 105, No. 2, 1993, p. 345-348.

    Research output: Contribution to journalArticle

    Nagle, Gregg T. ; van Heumen, Walter R A ; Knock, Susan L. ; Garcia, Anna T. ; McCullough, David A. ; Kurosky, Alexander. / Occurrence of a furin-like prohormone processing enzyme in Aplysia neuroendocrine bag cells. In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 1993 ; Vol. 105, No. 2. pp. 345-348.
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