TY - JOUR
T1 - Oleic acid nanoemulsion for nasal vaccination
T2 - Impact on adjuvanticity based immune response
AU - Sravanthi, Vemireddy
AU - Pallavi, M. C.Preethi
AU - Bonam, Srinivasa Reddy
AU - Sathyabama, Sathyaseelan
AU - Sampath Kumar, Halmuthur M.
N1 - Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Subunit vaccine formulations with enhanced potency are vital to improve immune response against weak antigens and also promote dose sparing of adjuvant and antigens that may cause undesirable side effects. Recently particulate delivery systems are under exploration to enhance antigen specific immunity. Oleic acid a potential biomaterial in terms of penetration is used for formulating nanoemulsion for nasal vaccination. In this study influence of oleic acid nanoemulsion on antigen uptake and role in dendritic cells activation is studied. Formulation conditions were optimized to produce a stable nano-emulsion at lowest surfactant concentration (2%). Penetration enhancement of oleic acid nanoemulsion was evaluated using cell uptake study by Fluorescein isothiocyanate conjugated OVA on A549 cell line. Here, we report strong class switched, high avidity humoral and cellular (cytokine) immune response elicited by vaccine delivery system with antigen and adjuvant. Flowcytometry analysis showed enhanced expression of activated dendritic cells on 45th day compared to soluble antigen. These data suggest that oleic acid formulation enhances penetration, sustain release of antigen and stimulates both cellular and humoral immune response.
AB - Subunit vaccine formulations with enhanced potency are vital to improve immune response against weak antigens and also promote dose sparing of adjuvant and antigens that may cause undesirable side effects. Recently particulate delivery systems are under exploration to enhance antigen specific immunity. Oleic acid a potential biomaterial in terms of penetration is used for formulating nanoemulsion for nasal vaccination. In this study influence of oleic acid nanoemulsion on antigen uptake and role in dendritic cells activation is studied. Formulation conditions were optimized to produce a stable nano-emulsion at lowest surfactant concentration (2%). Penetration enhancement of oleic acid nanoemulsion was evaluated using cell uptake study by Fluorescein isothiocyanate conjugated OVA on A549 cell line. Here, we report strong class switched, high avidity humoral and cellular (cytokine) immune response elicited by vaccine delivery system with antigen and adjuvant. Flowcytometry analysis showed enhanced expression of activated dendritic cells on 45th day compared to soluble antigen. These data suggest that oleic acid formulation enhances penetration, sustain release of antigen and stimulates both cellular and humoral immune response.
KW - Antibody class switch
KW - Dendritic cell activation
KW - Nasal vaccination
KW - Oleic acid nanoemulsion
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U2 - 10.1016/j.jddst.2015.05.007
DO - 10.1016/j.jddst.2015.05.007
M3 - Article
AN - SCOPUS:84941670986
SN - 1773-2247
VL - 28
SP - 56
EP - 63
JO - Journal of Drug Delivery Science and Technology
JF - Journal of Drug Delivery Science and Technology
ER -