Oligomerization of Uukuniemi virus nucleocapsid protein

Anna Katz, Alexander Freiberg, Vera Backström, Axel R. Schulz, Angelo Mateos, Liisa Holm, Ralf F. Pettersson, Antti Vaheri, Ramon Flick, Alexander Plyusnin

Research output: Contribution to journalArticle

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Abstract

Background. Uukuniemi virus (UUKV) belongs to the Phlebovirus genus in the family Bunyaviridae. As a non-pathogenic virus for humans UUKV has served as a safe model bunyavirus in a number of studies addressing fundamental questions such as organization and regulation of viral genes, genome replication, structure and assembly. The present study is focused on the oligomerization of the UUKV nucleocapsid (N) protein, which plays an important role in several steps of virus replication. The aim was to locate the domains involved in the N protein oligomerization and study the process in detail. Results. A set of experiments concentrating on the N- and C-termini of the protein was performed, first by completely or partially deleting putative N-N-interaction domains and then by introducing point mutations of amino acid residues. Mutagenesis strategy was based on the computer modeling of secondary and tertiary structure of the N protein. The N protein mutants were studied in chemical cross-linking, immunofluorescence, mammalian two-hybrid, minigenome, and virus-like particle-forming assays. The data showed that the oligomerization ability of UUKV-N protein depends on the presence of intact -helices on both termini of the N protein molecule and that a specific structure in the N-terminal region plays a crucial role in the N-N interaction(s). This structure is formed by two -helices, rich in amino acid residues with aromatic (W7, F10, W19, F27, F31) or long aliphatic (I14, I24) side chains. Furthermore, some of the N-terminal mutations (e.g. I14A, I24A, F31A) affected the N protein functionality both in mammalian two-hybrid and minigenome assays. Conclusions. UUKV-N protein has ability to form oligomers in chemical cross-linking and mammalian two-hybrid assays. In mutational analysis, some of the introduced single-point mutations abolished the N protein functionality both in mammalian two-hybrid and minigenome assays, suggesting that especially the N-terminal region of the UUKV-N protein is essential for the N-N interaction.

Original languageEnglish (US)
Article number187
JournalVirology Journal
Volume7
DOIs
StatePublished - 2010

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Uukuniemi virus
Nucleocapsid Proteins
Two-Hybrid System Techniques
Proteins
Point Mutation
Phlebovirus
Orthobunyavirus
Bunyaviridae
Amino Acids
Viral Genes
Viral Genome
Mutant Proteins
Virus Replication
Protein C
Tertiary Protein Structure
Mutagenesis
Virion
Fluorescent Antibody Technique
Viruses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Katz, A., Freiberg, A., Backström, V., Schulz, A. R., Mateos, A., Holm, L., ... Plyusnin, A. (2010). Oligomerization of Uukuniemi virus nucleocapsid protein. Virology Journal, 7, [187]. https://doi.org/10.1186/1743-422X-7-187

Oligomerization of Uukuniemi virus nucleocapsid protein. / Katz, Anna; Freiberg, Alexander; Backström, Vera; Schulz, Axel R.; Mateos, Angelo; Holm, Liisa; Pettersson, Ralf F.; Vaheri, Antti; Flick, Ramon; Plyusnin, Alexander.

In: Virology Journal, Vol. 7, 187, 2010.

Research output: Contribution to journalArticle

Katz, A, Freiberg, A, Backström, V, Schulz, AR, Mateos, A, Holm, L, Pettersson, RF, Vaheri, A, Flick, R & Plyusnin, A 2010, 'Oligomerization of Uukuniemi virus nucleocapsid protein', Virology Journal, vol. 7, 187. https://doi.org/10.1186/1743-422X-7-187
Katz, Anna ; Freiberg, Alexander ; Backström, Vera ; Schulz, Axel R. ; Mateos, Angelo ; Holm, Liisa ; Pettersson, Ralf F. ; Vaheri, Antti ; Flick, Ramon ; Plyusnin, Alexander. / Oligomerization of Uukuniemi virus nucleocapsid protein. In: Virology Journal. 2010 ; Vol. 7.
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AU - Backström, Vera

AU - Schulz, Axel R.

AU - Mateos, Angelo

AU - Holm, Liisa

AU - Pettersson, Ralf F.

AU - Vaheri, Antti

AU - Flick, Ramon

AU - Plyusnin, Alexander

PY - 2010

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N2 - Background. Uukuniemi virus (UUKV) belongs to the Phlebovirus genus in the family Bunyaviridae. As a non-pathogenic virus for humans UUKV has served as a safe model bunyavirus in a number of studies addressing fundamental questions such as organization and regulation of viral genes, genome replication, structure and assembly. The present study is focused on the oligomerization of the UUKV nucleocapsid (N) protein, which plays an important role in several steps of virus replication. The aim was to locate the domains involved in the N protein oligomerization and study the process in detail. Results. A set of experiments concentrating on the N- and C-termini of the protein was performed, first by completely or partially deleting putative N-N-interaction domains and then by introducing point mutations of amino acid residues. Mutagenesis strategy was based on the computer modeling of secondary and tertiary structure of the N protein. The N protein mutants were studied in chemical cross-linking, immunofluorescence, mammalian two-hybrid, minigenome, and virus-like particle-forming assays. The data showed that the oligomerization ability of UUKV-N protein depends on the presence of intact -helices on both termini of the N protein molecule and that a specific structure in the N-terminal region plays a crucial role in the N-N interaction(s). This structure is formed by two -helices, rich in amino acid residues with aromatic (W7, F10, W19, F27, F31) or long aliphatic (I14, I24) side chains. Furthermore, some of the N-terminal mutations (e.g. I14A, I24A, F31A) affected the N protein functionality both in mammalian two-hybrid and minigenome assays. Conclusions. UUKV-N protein has ability to form oligomers in chemical cross-linking and mammalian two-hybrid assays. In mutational analysis, some of the introduced single-point mutations abolished the N protein functionality both in mammalian two-hybrid and minigenome assays, suggesting that especially the N-terminal region of the UUKV-N protein is essential for the N-N interaction.

AB - Background. Uukuniemi virus (UUKV) belongs to the Phlebovirus genus in the family Bunyaviridae. As a non-pathogenic virus for humans UUKV has served as a safe model bunyavirus in a number of studies addressing fundamental questions such as organization and regulation of viral genes, genome replication, structure and assembly. The present study is focused on the oligomerization of the UUKV nucleocapsid (N) protein, which plays an important role in several steps of virus replication. The aim was to locate the domains involved in the N protein oligomerization and study the process in detail. Results. A set of experiments concentrating on the N- and C-termini of the protein was performed, first by completely or partially deleting putative N-N-interaction domains and then by introducing point mutations of amino acid residues. Mutagenesis strategy was based on the computer modeling of secondary and tertiary structure of the N protein. The N protein mutants were studied in chemical cross-linking, immunofluorescence, mammalian two-hybrid, minigenome, and virus-like particle-forming assays. The data showed that the oligomerization ability of UUKV-N protein depends on the presence of intact -helices on both termini of the N protein molecule and that a specific structure in the N-terminal region plays a crucial role in the N-N interaction(s). This structure is formed by two -helices, rich in amino acid residues with aromatic (W7, F10, W19, F27, F31) or long aliphatic (I14, I24) side chains. Furthermore, some of the N-terminal mutations (e.g. I14A, I24A, F31A) affected the N protein functionality both in mammalian two-hybrid and minigenome assays. Conclusions. UUKV-N protein has ability to form oligomers in chemical cross-linking and mammalian two-hybrid assays. In mutational analysis, some of the introduced single-point mutations abolished the N protein functionality both in mammalian two-hybrid and minigenome assays, suggesting that especially the N-terminal region of the UUKV-N protein is essential for the N-N interaction.

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