On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes

Lena Lewin, Mats Olof Mattsson, David K. Rassin, Åke Sellström

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The uptake of radioactive γ-aminobutyric acid (GABA) and d-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on this uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, we also used the P2-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for [3H]GABA and for d-[3H]aspartate with an increasing Vmax during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P2-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake of d-aspartate. We found no non-saturable uptake of d-aspartate.

Original languageEnglish (US)
Pages (from-to)333-337
Number of pages5
JournalNeurochemical Research
Volume17
Issue number4
DOIs
StatePublished - Apr 1992
Externally publishedYes

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Keywords

  • d-aspartate
  • GABA
  • glutamate
  • primary neuronal culture
  • rat synaptosomes
  • SKF 89976-A

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

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