On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes

Lena Lewin, Mats Olof Mattsson, David K. Rassin, Åke Sellström

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The uptake of radioactive γ-aminobutyric acid (GABA) and d-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on this uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, we also used the P2-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for [3H]GABA and for d-[3H]aspartate with an increasing Vmax during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P2-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake of d-aspartate. We found no non-saturable uptake of d-aspartate.

Original languageEnglish (US)
Pages (from-to)333-337
Number of pages5
JournalNeurochemical Research
Volume17
Issue number4
DOIs
StatePublished - Apr 1 1992

Keywords

  • GABA
  • SKF 89976-A
  • d-aspartate
  • glutamate
  • primary neuronal culture
  • rat synaptosomes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes'. Together they form a unique fingerprint.

  • Cite this