On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes

Yong Zhang, Owen Hamill

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

1. Mechanical stimulation of voltage-clamped Xenopus oocytes by inflation, aspiration, or local indentation failed to activate an increase in membrane conductance up to the point of causing visible oocyte damage. 2. The absence of mechanosensitivity is not due to the vitelline membrane, rapid MG channel adaptation or tension-sensitive recruitment of new membrane. 3. Membrane capacitance measurements indicate that the oocyte surface area is at least 5 times larger than that predicted assuming a smooth sphere. We propose that this excess membrane area provides an immediate reserve that can 'buffer' membrane tension changes and thus prevent MG channel activation. 4. High-resolution images of tightly sealed patches and patch capacitance measurements indicate a smooth membrane that is pulled flat and perpendicular across the inside of the pipette. Brief steps of pressure or suction cause rapid and reversible membrane flexing and MG channel activation. 5. We propose that changes in membrane geometry induced during cell growth and differentiation or as a consequence of specific physiological and pathological conditions may alter mechanosensitivity of a cell independently of the intrinsic properties of channel proteins.

Original languageEnglish (US)
Pages (from-to)101-115
Number of pages15
JournalJournal of Physiology
Volume523
Issue number1
StatePublished - Feb 15 2000

Fingerprint

Xenopus
Oocytes
Cell Membrane
Membranes
Vitelline Membrane
Oocyte Retrieval
Economic Inflation
Suction
Cell Differentiation
Buffers
Pressure
Growth

ASJC Scopus subject areas

  • Physiology

Cite this

On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes. / Zhang, Yong; Hamill, Owen.

In: Journal of Physiology, Vol. 523, No. 1, 15.02.2000, p. 101-115.

Research output: Contribution to journalArticle

@article{369f5620f0d645df9001d88b3bec8fd9,
title = "On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes",
abstract = "1. Mechanical stimulation of voltage-clamped Xenopus oocytes by inflation, aspiration, or local indentation failed to activate an increase in membrane conductance up to the point of causing visible oocyte damage. 2. The absence of mechanosensitivity is not due to the vitelline membrane, rapid MG channel adaptation or tension-sensitive recruitment of new membrane. 3. Membrane capacitance measurements indicate that the oocyte surface area is at least 5 times larger than that predicted assuming a smooth sphere. We propose that this excess membrane area provides an immediate reserve that can 'buffer' membrane tension changes and thus prevent MG channel activation. 4. High-resolution images of tightly sealed patches and patch capacitance measurements indicate a smooth membrane that is pulled flat and perpendicular across the inside of the pipette. Brief steps of pressure or suction cause rapid and reversible membrane flexing and MG channel activation. 5. We propose that changes in membrane geometry induced during cell growth and differentiation or as a consequence of specific physiological and pathological conditions may alter mechanosensitivity of a cell independently of the intrinsic properties of channel proteins.",
author = "Yong Zhang and Owen Hamill",
year = "2000",
month = "2",
day = "15",
language = "English (US)",
volume = "523",
pages = "101--115",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes

AU - Zhang, Yong

AU - Hamill, Owen

PY - 2000/2/15

Y1 - 2000/2/15

N2 - 1. Mechanical stimulation of voltage-clamped Xenopus oocytes by inflation, aspiration, or local indentation failed to activate an increase in membrane conductance up to the point of causing visible oocyte damage. 2. The absence of mechanosensitivity is not due to the vitelline membrane, rapid MG channel adaptation or tension-sensitive recruitment of new membrane. 3. Membrane capacitance measurements indicate that the oocyte surface area is at least 5 times larger than that predicted assuming a smooth sphere. We propose that this excess membrane area provides an immediate reserve that can 'buffer' membrane tension changes and thus prevent MG channel activation. 4. High-resolution images of tightly sealed patches and patch capacitance measurements indicate a smooth membrane that is pulled flat and perpendicular across the inside of the pipette. Brief steps of pressure or suction cause rapid and reversible membrane flexing and MG channel activation. 5. We propose that changes in membrane geometry induced during cell growth and differentiation or as a consequence of specific physiological and pathological conditions may alter mechanosensitivity of a cell independently of the intrinsic properties of channel proteins.

AB - 1. Mechanical stimulation of voltage-clamped Xenopus oocytes by inflation, aspiration, or local indentation failed to activate an increase in membrane conductance up to the point of causing visible oocyte damage. 2. The absence of mechanosensitivity is not due to the vitelline membrane, rapid MG channel adaptation or tension-sensitive recruitment of new membrane. 3. Membrane capacitance measurements indicate that the oocyte surface area is at least 5 times larger than that predicted assuming a smooth sphere. We propose that this excess membrane area provides an immediate reserve that can 'buffer' membrane tension changes and thus prevent MG channel activation. 4. High-resolution images of tightly sealed patches and patch capacitance measurements indicate a smooth membrane that is pulled flat and perpendicular across the inside of the pipette. Brief steps of pressure or suction cause rapid and reversible membrane flexing and MG channel activation. 5. We propose that changes in membrane geometry induced during cell growth and differentiation or as a consequence of specific physiological and pathological conditions may alter mechanosensitivity of a cell independently of the intrinsic properties of channel proteins.

UR - http://www.scopus.com/inward/record.url?scp=0034652418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034652418&partnerID=8YFLogxK

M3 - Article

C2 - 10673547

AN - SCOPUS:0034652418

VL - 523

SP - 101

EP - 115

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 1

ER -