On the inhibition of GABA release from a preparation of rat brain nerve-endings by SKF 89976-A, a GABA uptake inhibitor

Åke Sellström, David Rassin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fractions enriched in nerve-terminals were made in order to investigate the release of γ-aminobutyric acid (GABA). A P2-preparation made from whole rat brain was pre-labeled with [3H]GABA and thereafter incubated in high concentrations (1 mM) of the GABA uptake inhibitor SKF 89976-A. The nerve-terminals were thereafter incubated in depolarizing concentrations of KCl and finally the medium and nerve-terminals separated by a filtration procedure. The amount of [3H]GABA present on the filter and in the supernatant was measured by liquid scintillation counting and used to assess the release rate. SKF 89976-A reduced the KCl-induced efflux of [3H]GABA from the nerve-terminals. The inhibition was as effective against KCl-induced [3H]GABA efflux in the presence as well as in the absence of Ca2+. Our finding may demonstrate an inhibition of the inside-out activity of the GABA transporter.

Original languageEnglish (US)
Pages (from-to)189-192
Number of pages4
JournalNeuroscience Letters
Volume127
Issue number2
DOIs
StatePublished - Jun 24 1991

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GABA Uptake Inhibitors
Nerve Endings
gamma-Aminobutyric Acid
Brain
GABA Plasma Membrane Transport Proteins
Aminobutyrates
Scintillation Counting

Keywords

  • Nerve terminal
  • Release of SKF 89976-A
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

On the inhibition of GABA release from a preparation of rat brain nerve-endings by SKF 89976-A, a GABA uptake inhibitor. / Sellström, Åke; Rassin, David.

In: Neuroscience Letters, Vol. 127, No. 2, 24.06.1991, p. 189-192.

Research output: Contribution to journalArticle

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