On the mechanism of ATP induced shape changes in human erythrocyte membranes. I. The role of the spectrin complex

Michael Sheetz, S. J. Singer

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Human erythrocyte ghosts have been shown, by scanning electron microscopy, to undergo ATP-dependent shape changes. Under appropriate conditions the ghosts prepared from normal disk-shaped intact cells adopt a highly crenated shape, which in the presence of Mg-ATP at 37°C is slowly converted to the disk shape and eventually to the cup shape. These changes are not observed with other nucleotides or with 5'-adenylylimidodiphosphate. Anti-spectrin antibodies, incorporated along with the Mg-ATP into the ghosts in amounts less than equivalent to the spectrin, markedly accelerate the shape changes observed with the Mg-ATP alone. The Fab fragments of these antibodies, however, have no effect. The conclusion is that the structural effect produced by the ATP is promoted by the cross-linking of spectrin by its antibodies, and may therefore itself be some kind of polymerization or network formation involving the spectrin complex on the cytoplasmic face of the membrane. The factors that contribute to the shape of the ghost and of the intact erythrocyte are discussed in the light of these findings.

Original languageEnglish (US)
Pages (from-to)638-646
Number of pages9
JournalJournal of Cell Biology
Volume73
Issue number3
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

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Spectrin
Erythrocyte Membrane
Adenosine Triphosphate
Adenylyl Imidodiphosphate
Immunoglobulin Fab Fragments
Antibodies
Polymerization
Electron Scanning Microscopy
Anti-Idiotypic Antibodies
Nucleotides
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

Cite this

On the mechanism of ATP induced shape changes in human erythrocyte membranes. I. The role of the spectrin complex. / Sheetz, Michael; Singer, S. J.

In: Journal of Cell Biology, Vol. 73, No. 3, 01.01.1977, p. 638-646.

Research output: Contribution to journalArticle

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