On the negative staining of the protein crystal structure

Michael Sherman, E. V. Orlova, S. S. Terzyan, R. Kleine, N. A. Kiselev

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

It has been shown that in some cases negative staining reveals structure with details down to 0.4 nm in size, the nature of a protein playing a key role. Various stains seem to interact with different parts of a thermitase (a serine protease) molecule, which results in intensity changes in electron diffraction patterns.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalUltramicroscopy
Volume7
Issue number2
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

protease
Serine Proteases
staining
Electron diffraction
Diffraction patterns
Coloring Agents
diffraction patterns
electron diffraction
Crystal structure
proteins
Proteins
crystal structure
Molecules
molecules
thermitase
Peptide Hydrolases

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation

Cite this

Sherman, M., Orlova, E. V., Terzyan, S. S., Kleine, R., & Kiselev, N. A. (1981). On the negative staining of the protein crystal structure. Ultramicroscopy, 7(2), 131-137. https://doi.org/10.1016/0304-3991(81)90002-4

On the negative staining of the protein crystal structure. / Sherman, Michael; Orlova, E. V.; Terzyan, S. S.; Kleine, R.; Kiselev, N. A.

In: Ultramicroscopy, Vol. 7, No. 2, 1981, p. 131-137.

Research output: Contribution to journalArticle

Sherman, M, Orlova, EV, Terzyan, SS, Kleine, R & Kiselev, NA 1981, 'On the negative staining of the protein crystal structure', Ultramicroscopy, vol. 7, no. 2, pp. 131-137. https://doi.org/10.1016/0304-3991(81)90002-4
Sherman, Michael ; Orlova, E. V. ; Terzyan, S. S. ; Kleine, R. ; Kiselev, N. A. / On the negative staining of the protein crystal structure. In: Ultramicroscopy. 1981 ; Vol. 7, No. 2. pp. 131-137.
@article{a5d6b2716736463a91399f309ae942b8,
title = "On the negative staining of the protein crystal structure",
abstract = "It has been shown that in some cases negative staining reveals structure with details down to 0.4 nm in size, the nature of a protein playing a key role. Various stains seem to interact with different parts of a thermitase (a serine protease) molecule, which results in intensity changes in electron diffraction patterns.",
author = "Michael Sherman and Orlova, {E. V.} and Terzyan, {S. S.} and R. Kleine and Kiselev, {N. A.}",
year = "1981",
doi = "10.1016/0304-3991(81)90002-4",
language = "English (US)",
volume = "7",
pages = "131--137",
journal = "Ultramicroscopy",
issn = "0304-3991",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - On the negative staining of the protein crystal structure

AU - Sherman, Michael

AU - Orlova, E. V.

AU - Terzyan, S. S.

AU - Kleine, R.

AU - Kiselev, N. A.

PY - 1981

Y1 - 1981

N2 - It has been shown that in some cases negative staining reveals structure with details down to 0.4 nm in size, the nature of a protein playing a key role. Various stains seem to interact with different parts of a thermitase (a serine protease) molecule, which results in intensity changes in electron diffraction patterns.

AB - It has been shown that in some cases negative staining reveals structure with details down to 0.4 nm in size, the nature of a protein playing a key role. Various stains seem to interact with different parts of a thermitase (a serine protease) molecule, which results in intensity changes in electron diffraction patterns.

UR - http://www.scopus.com/inward/record.url?scp=0019764685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019764685&partnerID=8YFLogxK

U2 - 10.1016/0304-3991(81)90002-4

DO - 10.1016/0304-3991(81)90002-4

M3 - Article

C2 - 6175064

AN - SCOPUS:0019764685

VL - 7

SP - 131

EP - 137

JO - Ultramicroscopy

JF - Ultramicroscopy

SN - 0304-3991

IS - 2

ER -