Oncogene expression in reticuloendotheliosis virus-transformed lymphoid cell lines and avian tissues

N. K. Herzog, W. J. Bargmann, H. R. Bose

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The genome of reticuloendotheliosis virus (REV-T) contains a unique oncogene, designated v-rel, which is inserted into the env region. Employing a cloned rel DNA probe, a single 2.9- to 3.0-kilobase v-rel mRNA was identified in poly(A)+ RNA from REV-T-transformed lymphoid cell lines. A 4.0-kilobase rel-specific transcript corresponding to the cellular homolog of the v-rel oncogene was identified in MSB-1 cells, a herpesvirus-transformed lymphoid cell line. Cytodot hybridization was used to quantitate the levels of rel, c-rel, c-myc, c-myb, c-abl, c-fms, c-Ha-ras, c-Ki-ras, c-src, c-yes, c-mos, and c-sis mRNA in REV-T-transformed cells. The levels of rel transcription in REV-T-transformed cells were elevated only two to eightfold over levels found in the transformed immature avian lymphoid cell line MSB-1. The relatively modest levels of rel transcription in REV-T-transformed cells and the significant differences between the lengths of the v-rel and c-rel mRNA suggest that REV-T transformation is the result of the production of an altered rel protein. The c-rel proto-oncogene is expressed in all avian hematopoietic tissues but is not expressed at significant levels in brain and muscle. The transcription of other proto-oncogenes is not enhanced in REV-T-transformed lymphoid cell lines.

Original languageEnglish (US)
Pages (from-to)371-375
Number of pages5
JournalJournal of Virology
Volume57
Issue number1
StatePublished - 1986
Externally publishedYes

Fingerprint

Reticuloendotheliosis virus
rel Genes
Transformed Cell Line
oncogenes
Oncogenes
cell lines
Lymphocytes
proto-oncogenes
Messenger RNA
transcription (genetics)
T-lymphocytes
Proto-Oncogenes
Herpesviridae
DNA probes
DNA Probes
messenger RNA
hybridization
immatures
Genome
brain

ASJC Scopus subject areas

  • Immunology

Cite this

Oncogene expression in reticuloendotheliosis virus-transformed lymphoid cell lines and avian tissues. / Herzog, N. K.; Bargmann, W. J.; Bose, H. R.

In: Journal of Virology, Vol. 57, No. 1, 1986, p. 371-375.

Research output: Contribution to journalArticle

Herzog, N. K. ; Bargmann, W. J. ; Bose, H. R. / Oncogene expression in reticuloendotheliosis virus-transformed lymphoid cell lines and avian tissues. In: Journal of Virology. 1986 ; Vol. 57, No. 1. pp. 371-375.
@article{fa4f68a1a5f4485f997a28e08b594d68,
title = "Oncogene expression in reticuloendotheliosis virus-transformed lymphoid cell lines and avian tissues",
abstract = "The genome of reticuloendotheliosis virus (REV-T) contains a unique oncogene, designated v-rel, which is inserted into the env region. Employing a cloned rel DNA probe, a single 2.9- to 3.0-kilobase v-rel mRNA was identified in poly(A)+ RNA from REV-T-transformed lymphoid cell lines. A 4.0-kilobase rel-specific transcript corresponding to the cellular homolog of the v-rel oncogene was identified in MSB-1 cells, a herpesvirus-transformed lymphoid cell line. Cytodot hybridization was used to quantitate the levels of rel, c-rel, c-myc, c-myb, c-abl, c-fms, c-Ha-ras, c-Ki-ras, c-src, c-yes, c-mos, and c-sis mRNA in REV-T-transformed cells. The levels of rel transcription in REV-T-transformed cells were elevated only two to eightfold over levels found in the transformed immature avian lymphoid cell line MSB-1. The relatively modest levels of rel transcription in REV-T-transformed cells and the significant differences between the lengths of the v-rel and c-rel mRNA suggest that REV-T transformation is the result of the production of an altered rel protein. The c-rel proto-oncogene is expressed in all avian hematopoietic tissues but is not expressed at significant levels in brain and muscle. The transcription of other proto-oncogenes is not enhanced in REV-T-transformed lymphoid cell lines.",
author = "Herzog, {N. K.} and Bargmann, {W. J.} and Bose, {H. R.}",
year = "1986",
language = "English (US)",
volume = "57",
pages = "371--375",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Oncogene expression in reticuloendotheliosis virus-transformed lymphoid cell lines and avian tissues

AU - Herzog, N. K.

AU - Bargmann, W. J.

AU - Bose, H. R.

PY - 1986

Y1 - 1986

N2 - The genome of reticuloendotheliosis virus (REV-T) contains a unique oncogene, designated v-rel, which is inserted into the env region. Employing a cloned rel DNA probe, a single 2.9- to 3.0-kilobase v-rel mRNA was identified in poly(A)+ RNA from REV-T-transformed lymphoid cell lines. A 4.0-kilobase rel-specific transcript corresponding to the cellular homolog of the v-rel oncogene was identified in MSB-1 cells, a herpesvirus-transformed lymphoid cell line. Cytodot hybridization was used to quantitate the levels of rel, c-rel, c-myc, c-myb, c-abl, c-fms, c-Ha-ras, c-Ki-ras, c-src, c-yes, c-mos, and c-sis mRNA in REV-T-transformed cells. The levels of rel transcription in REV-T-transformed cells were elevated only two to eightfold over levels found in the transformed immature avian lymphoid cell line MSB-1. The relatively modest levels of rel transcription in REV-T-transformed cells and the significant differences between the lengths of the v-rel and c-rel mRNA suggest that REV-T transformation is the result of the production of an altered rel protein. The c-rel proto-oncogene is expressed in all avian hematopoietic tissues but is not expressed at significant levels in brain and muscle. The transcription of other proto-oncogenes is not enhanced in REV-T-transformed lymphoid cell lines.

AB - The genome of reticuloendotheliosis virus (REV-T) contains a unique oncogene, designated v-rel, which is inserted into the env region. Employing a cloned rel DNA probe, a single 2.9- to 3.0-kilobase v-rel mRNA was identified in poly(A)+ RNA from REV-T-transformed lymphoid cell lines. A 4.0-kilobase rel-specific transcript corresponding to the cellular homolog of the v-rel oncogene was identified in MSB-1 cells, a herpesvirus-transformed lymphoid cell line. Cytodot hybridization was used to quantitate the levels of rel, c-rel, c-myc, c-myb, c-abl, c-fms, c-Ha-ras, c-Ki-ras, c-src, c-yes, c-mos, and c-sis mRNA in REV-T-transformed cells. The levels of rel transcription in REV-T-transformed cells were elevated only two to eightfold over levels found in the transformed immature avian lymphoid cell line MSB-1. The relatively modest levels of rel transcription in REV-T-transformed cells and the significant differences between the lengths of the v-rel and c-rel mRNA suggest that REV-T transformation is the result of the production of an altered rel protein. The c-rel proto-oncogene is expressed in all avian hematopoietic tissues but is not expressed at significant levels in brain and muscle. The transcription of other proto-oncogenes is not enhanced in REV-T-transformed lymphoid cell lines.

UR - http://www.scopus.com/inward/record.url?scp=0022637864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022637864&partnerID=8YFLogxK

M3 - Article

VL - 57

SP - 371

EP - 375

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -