Opioid and tachykinin peptides, and their precursors and precursor-processing enzymes, in human cerebrospinal fluid

Danxia Liu, Chhabil Dass, George Wood, Dominic M. Desiderio

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Opioid and tachykinin neuropeptides, which were derived from two biological sources (intact, and released from their corresponding precursors by the action of human cerebrospinal fluid (CSF) neuropeptidases), were characterized in human CSF by using a combination of post-high-performance liquid chromatographic (HPLC) detection techniques. Peptides were separated using gradient and isocratic reversed-phase HPLC. Radioimmunoassay measured immunoreactivity corresponding to several different individual neuropeptides including methionine enkephalin, leucine enkephalin, substance P and β-endorphin. Commercial enzymes (trypsin, carboxypeptidase B) were used to release methionine- and leucine-enkephalin from precursors. Human CSF also served as a source of endogenous neuropeptidases. Mass spectrometry produced fragment ions that corroborated the amino acid sequence of methionine enkephalin and of substance P derived from both sources (intact, from precursors). These results demonstrated the presence of endogenous intact neuropeptides, several different neuropeptide-containing precursors and appropriate precursor-processing enzymes in human CSF for precursors of methionine enkephalin, leucine enkephalin, β-endorphin1-31 and substance P.

Original languageEnglish (US)
Pages (from-to)395-412
Number of pages18
JournalJournal of Chromatography A
Volume500
Issue numberC
DOIs
StatePublished - Feb 2 1990
Externally publishedYes

Fingerprint

Cerebrospinal fluid
Tachykinins
Methionine Enkephalin
Enzyme Precursors
Opioid Peptides
Neuropeptides
Opioid Analgesics
Cerebrospinal Fluid
Substance P
Leucine Enkephalin
Peptides
Enzymes
Processing
Carboxypeptidase B
Endorphins
Liquids
Trypsin
Radioimmunoassay
Mass spectrometry
Amino Acid Sequence

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Opioid and tachykinin peptides, and their precursors and precursor-processing enzymes, in human cerebrospinal fluid. / Liu, Danxia; Dass, Chhabil; Wood, George; Desiderio, Dominic M.

In: Journal of Chromatography A, Vol. 500, No. C, 02.02.1990, p. 395-412.

Research output: Contribution to journalArticle

Liu, Danxia ; Dass, Chhabil ; Wood, George ; Desiderio, Dominic M. / Opioid and tachykinin peptides, and their precursors and precursor-processing enzymes, in human cerebrospinal fluid. In: Journal of Chromatography A. 1990 ; Vol. 500, No. C. pp. 395-412.
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