Opposing roles of mitochondrial and nuclear PARP1 in the regulation of mitochondrial and nuclear DNA integrity: Implications for the regulation of mitochondrial function

Bartosz Szczesny, Attila Brunyanszki, Gabor Olah, Sankar Mitra, Csaba Szabo

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The positive role of PARP1 in regulation of various nuclear DNA transactions is well established. Although a mitochondrial localization of PARP1 has been suggested, its role in the maintenance of the mitochondrial DNA is currently unknown. Here we investigated the role of PARP1 in the repair of the mitochondrial DNA in the baseline and oxidative stress conditions. We used wild-type A549 cells or cells depleted of PARP1. Our data show that intra-mitochondrial PARP1 interacts with a key mitochondrial-specific DNA base excision repair (BER) enzymes, namely EXOG and DNA polymerase gamma (Polγ), which under oxidative stress become poly(ADP-ribose)lated (PARylated). Interaction between mitochondrial BER enzymes was significantly affected in the presence of PARP1. Moreover, the repair of the oxidative-induced damage to the mitochondrial DNA in PARP1-depleted cells was found to be more robust compared to control counterpart. In addition, mitochondrial biogenesis was enhanced in PARP1-depleted cells, including mitochondrial DNA copy number and mitochondrial membrane potential. This observation was further confirmed by analysis of lung tissue isolated from WT and PARP1 KO mice. In summary, we conclude that mitochondrial PARP1, in opposite to nuclear PARP1, exerts a negative effect on several mitochondrial-specific transactions including the repair of the mitochondrial DNA.

Original languageEnglish (US)
Pages (from-to)13161-13173
Number of pages13
JournalNucleic Acids Research
Volume42
Issue number21
DOIs
StatePublished - Dec 1 2014

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Mitochondrial DNA
DNA Repair
Oxidative Stress
Poly Adenosine Diphosphate Ribose
Mitochondrial Membrane Potential
Organelle Biogenesis
Enzymes
Maintenance
Lung
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Opposing roles of mitochondrial and nuclear PARP1 in the regulation of mitochondrial and nuclear DNA integrity : Implications for the regulation of mitochondrial function. / Szczesny, Bartosz; Brunyanszki, Attila; Olah, Gabor; Mitra, Sankar; Szabo, Csaba.

In: Nucleic Acids Research, Vol. 42, No. 21, 01.12.2014, p. 13161-13173.

Research output: Contribution to journalArticle

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AU - Szabo, Csaba

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