Purpose: We investigated in a surgical rat model of vascular injury the potential role of the peroxynitrite - poly(ADPribose) polymerase (PARP) pathway in inflammatory response and apoptosis induction after vascular gamma irradiation. Methods: Male Sprague-Dawley rats underwent left carotid endarterectomy with removal of intima: control (n = 10) and were irradiated with 15 Gray (n = 13) or 20 Gray (n = 10) postoperatively and compared with sham-operated rats (n = 10). Additional animals were solely irradiated with 15 Gy (n = 10) and with 20 Gy (n = 10) to distinguish between primary effects of vascular injury and secondary effects due to irradiation. Results: After 21 days, neointima formation was significantly suppressed after irradiation (control: 0.07 mm2 ± 0.04 mm2, 15 Gy: 0.003 mm 2 ± 0.004 mm2, 20 Gy: 0.001 mm2 ± 0.0006 mm2, P< 0.0001). However, a significant inflammation of the vessel wall with focal wall necrosis was detected (control: 0.2 ± 0.15, 15 Gy: 0.82 ± 1.2, 20 Gy: 1.25 ± 0.86, P= 0.003). Immunohistochemistry showed significant staining for nitrotyrosine, poly(ADP-ribose) and nuclear translocation of apoptosis-inducing factor in the neointima of the control group. In the irradiated groups these stainings were significantly higher in the media and adventitia compared to the non-irradiated groups. Conclusion: Activation of the peroxynitrite-PARP pathway was demonstrated during neointima proliferation in a rat model of surgical vascular injury. Vascular irradiation suppressed neointima formation, but induced significant activation of the peroxynitrite - PARP pathway in the outer vessel wall layers concomitant to inflammation and focal wall necrosis. This may contribute to adverse effects of vascular irradiation such as fibrosis and constrictive remodeling.
- Peroxynitrite-PARP pathway
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine