TY - JOUR
T1 - Optical biosensing of markers of mucosal inflammation
AU - Covarrubias-Zambrano, Obdulia
AU - Motamedi, Massoud
AU - Ameredes, Bill T.
AU - Tian, Bing
AU - Calhoun, William J.
AU - Zhao, Yingxin
AU - Brasier, Allan R.
AU - Kalubowilage, Madumali
AU - Malalasekera, Aruni P.
AU - Yapa, Asanka S.
AU - Wang, Hongwang
AU - Culbertson, Christopher T.
AU - Troyer, Deryl L.
AU - Bossmann, Stefan H.
N1 - Funding Information:
This work was funded by NSF (CBET 1804416 (SHB/MM/CK), 1826982 (SHB) and EFMA 2032751 (SHB/MM/BTA) and EFMA 2129617 (SHB/MM/CK); NIH (The NHLBI Severe Asthma Research Project (SARP) HL-69130 (WJC); The UTMB Clinical and Translational Science Award (CTSA) NIH/NCATS 8UL1TR000071 (ARB/WJC); PO1 AI068865 (ARB)); UTMB Sealy Center for Molecular Medicine (SCMM) (ARB/BT/YZ); The Brown Foundation (BTA); The University of Kansas Cancer Center (SHB); and Johnson Cancer Center at Kansas State (SHB).
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2022/2
Y1 - 2022/2
N2 - We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease.
AB - We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease.
KW - Iron/iron oxide core/shell nanoparticle
KW - Lung inflammation
KW - Nanodiagnostics
KW - Nanomedicine
KW - Optical biosensor
UR - https://www.scopus.com/pages/publications/85120877637
UR - https://www.scopus.com/pages/publications/85120877637#tab=citedBy
U2 - 10.1016/j.nano.2021.102476
DO - 10.1016/j.nano.2021.102476
M3 - Article
C2 - 34743019
AN - SCOPUS:85120877637
SN - 1549-9634
VL - 40
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
M1 - 102476
ER -