Optical coherence tomography for assessment of microbicide safety in a small animal model

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sensitive imaging techniques for small animals are needed to assess drug toxicity in preclinical studies. Optical coherence tomography (OCT) provides a noninvasive tool for high-resolution, depth-resolved visualization of drug-induced changes in tissue morphology. In a mouse model, we utilize OCT to assess vaginal tissue integrity following the application of topical microbicides (drugs used to prevent infection). Mice are challenged with herpes simplex virus-2 (HSV-2) to determine the correlation of tissue damage as quantified by OCT to increased susceptibility. The microbicide benzalkonium chloride (BZK) (0.02, 0.2, or 2%) or phosphate buffered saline control is administered intravaginally. In vivo OCT imaging and collection of tissue samples are performed after treatment. A quantitative OCT scoring system is applied to assess epithelial damage, and the results are compared with those of histology. A separate group of mice are treated similarly then challenged with HSV-2. Epithelial morphology quantified noninvasively by OCT and histology are dose-dependent (p < 0.0001). The OCT scoring system detected a significant increase in epithelial damage with increasing BZK concentration (p < 0.0001). These results paralleled an increase in HSV-2 susceptibility (p < 0.005). OCT can be used as a noninvasive tool to assess topical drug toxicity in a small animal model with potential to predict increased susceptibility to vaginal infection.

Original languageEnglish (US)
Article number046010
JournalJournal of Biomedical Optics
Volume18
Issue number4
DOIs
StatePublished - Apr 2013

Fingerprint

animal models
Optical tomography
Anti-Infective Agents
safety
Animals
tomography
drugs
viruses
Viruses
Tissue
Benzalkonium Compounds
mice
Histology
histology
scoring
infectious diseases
damage
magnetic permeability
toxicity
Pharmaceutical Preparations

Keywords

  • benzalkonium chloride
  • damage
  • drug
  • microbicides
  • mouse
  • optical coherence tomography
  • safety
  • toxicity
  • vaginal tissue

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

@article{d8f6882c633d4a179a0e06e389186bda,
title = "Optical coherence tomography for assessment of microbicide safety in a small animal model",
abstract = "Sensitive imaging techniques for small animals are needed to assess drug toxicity in preclinical studies. Optical coherence tomography (OCT) provides a noninvasive tool for high-resolution, depth-resolved visualization of drug-induced changes in tissue morphology. In a mouse model, we utilize OCT to assess vaginal tissue integrity following the application of topical microbicides (drugs used to prevent infection). Mice are challenged with herpes simplex virus-2 (HSV-2) to determine the correlation of tissue damage as quantified by OCT to increased susceptibility. The microbicide benzalkonium chloride (BZK) (0.02, 0.2, or 2{\%}) or phosphate buffered saline control is administered intravaginally. In vivo OCT imaging and collection of tissue samples are performed after treatment. A quantitative OCT scoring system is applied to assess epithelial damage, and the results are compared with those of histology. A separate group of mice are treated similarly then challenged with HSV-2. Epithelial morphology quantified noninvasively by OCT and histology are dose-dependent (p < 0.0001). The OCT scoring system detected a significant increase in epithelial damage with increasing BZK concentration (p < 0.0001). These results paralleled an increase in HSV-2 susceptibility (p < 0.005). OCT can be used as a noninvasive tool to assess topical drug toxicity in a small animal model with potential to predict increased susceptibility to vaginal infection.",
keywords = "benzalkonium chloride, damage, drug, microbicides, mouse, optical coherence tomography, safety, toxicity, vaginal tissue",
author = "Bell, {Brent A.} and Kathleen Vincent and Nigel Bourne and Gracie Vargas and Massoud Motamedi",
year = "2013",
month = "4",
doi = "10.1117/1.JBO.18.4.046010",
language = "English (US)",
volume = "18",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "4",

}

TY - JOUR

T1 - Optical coherence tomography for assessment of microbicide safety in a small animal model

AU - Bell, Brent A.

AU - Vincent, Kathleen

AU - Bourne, Nigel

AU - Vargas, Gracie

AU - Motamedi, Massoud

PY - 2013/4

Y1 - 2013/4

N2 - Sensitive imaging techniques for small animals are needed to assess drug toxicity in preclinical studies. Optical coherence tomography (OCT) provides a noninvasive tool for high-resolution, depth-resolved visualization of drug-induced changes in tissue morphology. In a mouse model, we utilize OCT to assess vaginal tissue integrity following the application of topical microbicides (drugs used to prevent infection). Mice are challenged with herpes simplex virus-2 (HSV-2) to determine the correlation of tissue damage as quantified by OCT to increased susceptibility. The microbicide benzalkonium chloride (BZK) (0.02, 0.2, or 2%) or phosphate buffered saline control is administered intravaginally. In vivo OCT imaging and collection of tissue samples are performed after treatment. A quantitative OCT scoring system is applied to assess epithelial damage, and the results are compared with those of histology. A separate group of mice are treated similarly then challenged with HSV-2. Epithelial morphology quantified noninvasively by OCT and histology are dose-dependent (p < 0.0001). The OCT scoring system detected a significant increase in epithelial damage with increasing BZK concentration (p < 0.0001). These results paralleled an increase in HSV-2 susceptibility (p < 0.005). OCT can be used as a noninvasive tool to assess topical drug toxicity in a small animal model with potential to predict increased susceptibility to vaginal infection.

AB - Sensitive imaging techniques for small animals are needed to assess drug toxicity in preclinical studies. Optical coherence tomography (OCT) provides a noninvasive tool for high-resolution, depth-resolved visualization of drug-induced changes in tissue morphology. In a mouse model, we utilize OCT to assess vaginal tissue integrity following the application of topical microbicides (drugs used to prevent infection). Mice are challenged with herpes simplex virus-2 (HSV-2) to determine the correlation of tissue damage as quantified by OCT to increased susceptibility. The microbicide benzalkonium chloride (BZK) (0.02, 0.2, or 2%) or phosphate buffered saline control is administered intravaginally. In vivo OCT imaging and collection of tissue samples are performed after treatment. A quantitative OCT scoring system is applied to assess epithelial damage, and the results are compared with those of histology. A separate group of mice are treated similarly then challenged with HSV-2. Epithelial morphology quantified noninvasively by OCT and histology are dose-dependent (p < 0.0001). The OCT scoring system detected a significant increase in epithelial damage with increasing BZK concentration (p < 0.0001). These results paralleled an increase in HSV-2 susceptibility (p < 0.005). OCT can be used as a noninvasive tool to assess topical drug toxicity in a small animal model with potential to predict increased susceptibility to vaginal infection.

KW - benzalkonium chloride

KW - damage

KW - drug

KW - microbicides

KW - mouse

KW - optical coherence tomography

KW - safety

KW - toxicity

KW - vaginal tissue

UR - http://www.scopus.com/inward/record.url?scp=84884171414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884171414&partnerID=8YFLogxK

U2 - 10.1117/1.JBO.18.4.046010

DO - 10.1117/1.JBO.18.4.046010

M3 - Article

VL - 18

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 4

M1 - 046010

ER -