Optimal drug and gene delivery in cancer cells by ultrasound-induced cavitation

Irina V. Larina, B. Mark Evers, Rinat Esenaliev

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Background: Lack of efficient drug and gene delivery is one of the major problems of cancer chemo- and bio-therapy. This work is focused on optimization of ultrasound-induced delivery of model macromolecular anti-cancer drugs and DNA in human cancer cells. Materials and Methods: FITC dextrans simulated antisense oligonucleotides (10 kDa), antibodies (70 kDa), and genes (2000 kDa). Optimization of ultrasound frequency, intensity, duty cycle, time of irradiation, and concentration of Optison (ultrasound contrast agent) was performed. Results: Optimal parameters provided in the MCF7 cell line 73.5±3.3%, 72.7±0.9%, and 62.7±2.1% delivery of 10-kDa, 70-kDa, and 2000-kDa macromolecules, respectively, 36.7±4.9% of cell transfection, while dead cell count was only 13.5±1.6%. Statistically significant drug delivery and transfection was obtained in all tested cell lines. Conclusion: These results suggest that optimized treatment parameters provide efficient drug and gene delivery in cancer cells and could be used for further in vivo and in vitro experiments.

Original languageEnglish (US)
Pages (from-to)149-156
Number of pages8
JournalAnticancer Research
Volume25
Issue number1 A
StatePublished - Jan 2005

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Pharmaceutical Preparations
Genes
Transfection
Neoplasms
Cell Line
Antisense Oligonucleotides
MCF-7 Cells
Contrast Media
Cell Count
Antibodies
DNA
Therapeutics
FS 069
fluorescein isothiocyanate dextran
In Vitro Techniques

Keywords

  • Biotherapy
  • Cavitation
  • Drug delivery
  • Gene therapy
  • Transfection
  • Ultrasound

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Optimal drug and gene delivery in cancer cells by ultrasound-induced cavitation. / Larina, Irina V.; Evers, B. Mark; Esenaliev, Rinat.

In: Anticancer Research, Vol. 25, No. 1 A, 01.2005, p. 149-156.

Research output: Contribution to journalArticle

Larina, Irina V. ; Evers, B. Mark ; Esenaliev, Rinat. / Optimal drug and gene delivery in cancer cells by ultrasound-induced cavitation. In: Anticancer Research. 2005 ; Vol. 25, No. 1 A. pp. 149-156.
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