TY - JOUR
T1 - Optimization and Characterization of a Bone Culture Model to Study Prostate Cancer Bone Metastasis
AU - Wu, Yi Hsuan
AU - Gugala, Zbigniew
AU - Barry, Megan M.
AU - Shen, Yichao
AU - Dasgupta, Subhamoy
AU - Wang, Hai
N1 - Publisher Copyright:
©2022 American Association for Cancer Research.
PY - 2022/8
Y1 - 2022/8
N2 - Nearly 90% of patients with advanced prostate cancer manifest bone metastases. Distinct from the osteolytic metastasis mostly observed in other cancer types, prostate cancer bone metastasis is typically more osteoblastic, which is relatively understudied due to the lack of reliable and efficient models to resemble the indolent cellular growth and complexity of metastatic progression. In our previous studies, we developed bone-in-culture array (BICA) to primarily model the osteoblastinvolved, pre-osteolytic stage of breast cancer bone metastasis. Given that the progression of prostate cancer bone metastasis is largely osteoblastic, it is reasonable to speculate that the original BICA model can be adjusted to investigate prostate cancer bone metastases. In this study, we refined BICA by reducing the surgical labor and improving its reproducibility and capacity. The optimized BICA can successfully recapitulate important features of prostate cancer bone metastasis such as the osteoblastic phenotype, indolent growth, cancer–niche interactions, and response to hormones. Our efforts address the longstanding need for reliable and efficient models to study prostate cancer bone metastasis.
AB - Nearly 90% of patients with advanced prostate cancer manifest bone metastases. Distinct from the osteolytic metastasis mostly observed in other cancer types, prostate cancer bone metastasis is typically more osteoblastic, which is relatively understudied due to the lack of reliable and efficient models to resemble the indolent cellular growth and complexity of metastatic progression. In our previous studies, we developed bone-in-culture array (BICA) to primarily model the osteoblastinvolved, pre-osteolytic stage of breast cancer bone metastasis. Given that the progression of prostate cancer bone metastasis is largely osteoblastic, it is reasonable to speculate that the original BICA model can be adjusted to investigate prostate cancer bone metastases. In this study, we refined BICA by reducing the surgical labor and improving its reproducibility and capacity. The optimized BICA can successfully recapitulate important features of prostate cancer bone metastasis such as the osteoblastic phenotype, indolent growth, cancer–niche interactions, and response to hormones. Our efforts address the longstanding need for reliable and efficient models to study prostate cancer bone metastasis.
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U2 - 10.1158/1535-7163.MCT-21-0684
DO - 10.1158/1535-7163.MCT-21-0684
M3 - Article
C2 - 35666809
AN - SCOPUS:85135597874
SN - 1535-7163
VL - 21
SP - 1360
EP - 1408
JO - Molecular Cancer Therapeutics
JF - Molecular Cancer Therapeutics
IS - 8
ER -