Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines

A. R. Brasier, J. E. Tate, J. F. Habener

Research output: Contribution to journalArticle

550 Citations (Scopus)

Abstract

The enzymatic activity of firefly luciferase provides a sensitive, rapid means to assay transcriptional activity of regulated activation sequences of DNA when fused to the protein coding sequence of the luciferase gene. We have developed several modification of the luciferase assay and have characterized certain parameters of the assay, resulting in optimization of conditions for the preparation and storage of cell lysates and establishment of substrate concentrations. These findings should be useful to investigators interested in applying the luciferase assay to studies of the transcriptional activity of test DNAs incorporating the luciferase reporter.

Original languageEnglish (US)
Pages (from-to)1116-1122
Number of pages7
JournalBioTechniques
Volume7
Issue number10
StatePublished - 1989
Externally publishedYes

Fingerprint

Firefly Luciferases
Luciferases
Reporter Genes
Assays
Genes
Cells
Cell Line
DNA
Chemical activation
Research Personnel
Substrates
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Brasier, A. R., Tate, J. E., & Habener, J. F. (1989). Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines. BioTechniques, 7(10), 1116-1122.

Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines. / Brasier, A. R.; Tate, J. E.; Habener, J. F.

In: BioTechniques, Vol. 7, No. 10, 1989, p. 1116-1122.

Research output: Contribution to journalArticle

Brasier, AR, Tate, JE & Habener, JF 1989, 'Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines', BioTechniques, vol. 7, no. 10, pp. 1116-1122.
Brasier, A. R. ; Tate, J. E. ; Habener, J. F. / Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines. In: BioTechniques. 1989 ; Vol. 7, No. 10. pp. 1116-1122.
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