Abstract
An otoacoustic transducer capable of detecting wide-band ultrasonic profiles at the site of pulsed laser irradiation was developed and applied in imaging various stages of squamous cell carcinoma produced in a hamster cheek pouch model. Imaging was performed using 12-nanosecond Nd:YAG laser pulses (FWHM) at the wavelength of 532 nm, which provided sufficient depth of monitoring (2 mm), significant tissue contrast and 15-μm in depth resolution. The opto-acoustic images depict distinct tissue layers in control (normal) animals and heterogeneous structureless tissue in pre-cancer and cancer stages. Pronounced changes in subsurface tissue structures that occur in the course of cancer development were not detected upon gross examination. Optoacoustic images of later stages in cancer development presented exophytic or irregular profiles on the surfaces, possibly associated with enhanced microvascularization. The changes associated with benign inflammation yielded optoacoustic images different from those depicting cancer progression. The experimental results suggest that the optoacoustic images displaying layered tissue structure on the basis of variations in optical absorption and scattering may be utilized for early detection and staging of superficial mucosa cancer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 385-396 |
| Number of pages | 12 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 3597 |
| State | Published - Dec 1 1999 |
| Event | Proceedings of the 1999 Optical Tomography and Spectroscopy of Tissue III - San Jose, CA, USA Duration: Jan 24 1999 → Jan 28 1999 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering