Optoacoustic 3D whole-body tomography: Experiments in nude mice

Hans Peter Brecht, Richard Su, Matt Fronheiser, Sergey A. Ermilov, Andre Conjusteau, Anton Liopo, Massoud Motamedi, Alexander A. Oraevsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

15 Citations (Scopus)

Abstract

We developed a 3D whole-body optoacoustic tomography system for applications in preclinical research on mice. The system is capable of generating images with resolution better than 0.6 mm. Two pulsed lasers, an Alexandrite laser operating at 755 nm and a Nd:YAG laser operating at 532 nm and 1064nm were used for light delivery. The tomographic images were obtained while the objects of study (phantoms or mice) were rotated within a sphere outlined by a concave arc-shaped array of 64 piezo-composite transducers. During the scan, the mouse was illuminated orthogonally to the array with two wide beams of light from a bifurcated fiber bundle. Illumination at 532 nm showed superficial vasculature, but limited penetration depth at this wavelength prevented the detection of deeper structures. Illumination at 755 and 1064 nm showed organs and blood vessels, respectively. Filtering of the optoacoustic signals using high frequency enhancing wavelets further emphasized the smaller blood vessels.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7177
DOIs
StatePublished - 2009
EventPhotons Plus Ultrasound: Imaging and Sensing 2009 - San Jose, CA, United States
Duration: Jan 25 2009Jan 28 2009

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2009
CountryUnited States
CitySan Jose, CA
Period1/25/091/28/09

Fingerprint

Photoacoustic effect
Tomography
mice
Mouse
tomography
Blood Vessels
blood vessels
Blood vessels
Illumination
Lighting
illumination
alexandrite
Experiment
Nd:YAG Laser
Lasers
Pulsed Laser
Experiments
Fiber Bundle
Phantom
Pulsed lasers

Keywords

  • Blood
  • Functional imaging
  • Image reconstruction
  • Optoacoustics
  • Small animal imaging
  • Vasculature

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Brecht, H. P., Su, R., Fronheiser, M., Ermilov, S. A., Conjusteau, A., Liopo, A., ... Oraevsky, A. A. (2009). Optoacoustic 3D whole-body tomography: Experiments in nude mice. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7177). [71770E] https://doi.org/10.1117/12.812319

Optoacoustic 3D whole-body tomography : Experiments in nude mice. / Brecht, Hans Peter; Su, Richard; Fronheiser, Matt; Ermilov, Sergey A.; Conjusteau, Andre; Liopo, Anton; Motamedi, Massoud; Oraevsky, Alexander A.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7177 2009. 71770E.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Brecht, HP, Su, R, Fronheiser, M, Ermilov, SA, Conjusteau, A, Liopo, A, Motamedi, M & Oraevsky, AA 2009, Optoacoustic 3D whole-body tomography: Experiments in nude mice. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7177, 71770E, Photons Plus Ultrasound: Imaging and Sensing 2009, San Jose, CA, United States, 1/25/09. https://doi.org/10.1117/12.812319
Brecht HP, Su R, Fronheiser M, Ermilov SA, Conjusteau A, Liopo A et al. Optoacoustic 3D whole-body tomography: Experiments in nude mice. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7177. 2009. 71770E https://doi.org/10.1117/12.812319
Brecht, Hans Peter ; Su, Richard ; Fronheiser, Matt ; Ermilov, Sergey A. ; Conjusteau, Andre ; Liopo, Anton ; Motamedi, Massoud ; Oraevsky, Alexander A. / Optoacoustic 3D whole-body tomography : Experiments in nude mice. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7177 2009.
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