Optoacoustic 3D whole-body tomography: Experiments in nude mice

Hans Peter Brecht, Richard Su, Matt Fronheiser, Sergey A. Ermilov, Andre Conjusteau, Anton Liopo, Massoud Motamedi, Alexander A. Oraevsky

Research output: Contribution to journalConference article

16 Scopus citations

Abstract

We developed a 3D whole-body optoacoustic tomography system for applications in preclinical research on mice. The system is capable of generating images with resolution better than 0.6 mm. Two pulsed lasers, an Alexandrite laser operating at 755 nm and a Nd:YAG laser operating at 532 nm and 1064nm were used for light delivery. The tomographic images were obtained while the objects of study (phantoms or mice) were rotated within a sphere outlined by a concave arc-shaped array of 64 piezo-composite transducers. During the scan, the mouse was illuminated orthogonally to the array with two wide beams of light from a bifurcated fiber bundle. Illumination at 532 nm showed superficial vasculature, but limited penetration depth at this wavelength prevented the detection of deeper structures. Illumination at 755 and 1064 nm showed organs and blood vessels, respectively. Filtering of the optoacoustic signals using high frequency enhancing wavelets further emphasized the smaller blood vessels.

Original languageEnglish (US)
Article number71770E
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7177
DOIs
StatePublished - Jun 8 2009
EventPhotons Plus Ultrasound: Imaging and Sensing 2009 - San Jose, CA, United States
Duration: Jan 25 2009Jan 28 2009

Keywords

  • Blood
  • Functional imaging
  • Image reconstruction
  • Optoacoustics
  • Small animal imaging
  • Vasculature

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Brecht, H. P., Su, R., Fronheiser, M., Ermilov, S. A., Conjusteau, A., Liopo, A., Motamedi, M., & Oraevsky, A. A. (2009). Optoacoustic 3D whole-body tomography: Experiments in nude mice. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7177, [71770E]. https://doi.org/10.1117/12.812319