Optoacoustic imaging of gold nanoparticles targeted to breast cancer cells

Mohammad Eghtedari, Massoud Motamedi, Vsevolod Popov, Nicholas A. Kotov, Alexander A. Oraevsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

15 Citations (Scopus)

Abstract

Optoacoustic Tomography (OAT) is a rapidly growing technology that enables noninvasive deep imaging of biological tissues based on their light absorption. In OAT, the interaction of a pulsed laser with tissue increases the temperature of the absorbing components in a confined volume of tissue. Rapid perturbation of the temperature (<1°C) deep within tissue produces weak acoustic waves that easily travel to the surface of the tissue with minor attenuation. Abnormal angiogenesis in a malignant tumor, that increases its blood content, makes a native contrast for optoacoustic imaging; however, the application of OAT for early detection of malignant tumors requires the enhancement of optoacoustic signals originated from tumor by using an exogenous contrast agent. Due to their strong absorption, we have used gold nanoparticles (NP) as a contrast agent. 40nm spherical gold nanoparticles were attached to monoclonal antibody to target cell surface of breast cancer cells. The targeted cancer cells were implanted at depth of 5-6cm within a gelatinous object that optically resembles human breast. Experimental sensitivity measurements along with theoretical analysis showed that our optoacoustic imaging system is capable of detecting a phantom breast tumor with the volume of 0.15ml, which is composed of 25 million NP-targeted cancer cells, at a depth of 5 centimeters in vitro.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsA.A. Oraevsky, L.V. Wang
Pages21-28
Number of pages8
Volume5
Edition9
DOIs
StatePublished - 2004
EventPhotons Plus Ultrasound: Imaging and Sensing - San Jose, CA, United States
Duration: Jan 25 2004Jan 26 2004

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing
CountryUnited States
CitySan Jose, CA
Period1/25/041/26/04

Fingerprint

Photoacoustic effect
Gold
Cells
Nanoparticles
Imaging techniques
Tissue
Tumors
Tomography
Monoclonal antibodies
Pulsed lasers
Imaging systems
Light absorption
Blood
Acoustic waves
Temperature

Keywords

  • Cancer
  • Gold Nanoparticle
  • Herceptin
  • Molecular Contrast
  • Optoacoustic Imaging
  • Sensitivity
  • Targeting

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Eghtedari, M., Motamedi, M., Popov, V., Kotov, N. A., & Oraevsky, A. A. (2004). Optoacoustic imaging of gold nanoparticles targeted to breast cancer cells. In A. A. Oraevsky, & L. V. Wang (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (9 ed., Vol. 5, pp. 21-28). [06] https://doi.org/10.1117/12.534464

Optoacoustic imaging of gold nanoparticles targeted to breast cancer cells. / Eghtedari, Mohammad; Motamedi, Massoud; Popov, Vsevolod; Kotov, Nicholas A.; Oraevsky, Alexander A.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / A.A. Oraevsky; L.V. Wang. Vol. 5 9. ed. 2004. p. 21-28 06.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Eghtedari, M, Motamedi, M, Popov, V, Kotov, NA & Oraevsky, AA 2004, Optoacoustic imaging of gold nanoparticles targeted to breast cancer cells. in AA Oraevsky & LV Wang (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 9 edn, vol. 5, 06, pp. 21-28, Photons Plus Ultrasound: Imaging and Sensing, San Jose, CA, United States, 1/25/04. https://doi.org/10.1117/12.534464
Eghtedari M, Motamedi M, Popov V, Kotov NA, Oraevsky AA. Optoacoustic imaging of gold nanoparticles targeted to breast cancer cells. In Oraevsky AA, Wang LV, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 9 ed. Vol. 5. 2004. p. 21-28. 06 https://doi.org/10.1117/12.534464
Eghtedari, Mohammad ; Motamedi, Massoud ; Popov, Vsevolod ; Kotov, Nicholas A. ; Oraevsky, Alexander A. / Optoacoustic imaging of gold nanoparticles targeted to breast cancer cells. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / A.A. Oraevsky ; L.V. Wang. Vol. 5 9. ed. 2004. pp. 21-28
@inproceedings{4d65c3a8271f470b985b68c645bea1f6,
title = "Optoacoustic imaging of gold nanoparticles targeted to breast cancer cells",
abstract = "Optoacoustic Tomography (OAT) is a rapidly growing technology that enables noninvasive deep imaging of biological tissues based on their light absorption. In OAT, the interaction of a pulsed laser with tissue increases the temperature of the absorbing components in a confined volume of tissue. Rapid perturbation of the temperature (<1°C) deep within tissue produces weak acoustic waves that easily travel to the surface of the tissue with minor attenuation. Abnormal angiogenesis in a malignant tumor, that increases its blood content, makes a native contrast for optoacoustic imaging; however, the application of OAT for early detection of malignant tumors requires the enhancement of optoacoustic signals originated from tumor by using an exogenous contrast agent. Due to their strong absorption, we have used gold nanoparticles (NP) as a contrast agent. 40nm spherical gold nanoparticles were attached to monoclonal antibody to target cell surface of breast cancer cells. The targeted cancer cells were implanted at depth of 5-6cm within a gelatinous object that optically resembles human breast. Experimental sensitivity measurements along with theoretical analysis showed that our optoacoustic imaging system is capable of detecting a phantom breast tumor with the volume of 0.15ml, which is composed of 25 million NP-targeted cancer cells, at a depth of 5 centimeters in vitro.",
keywords = "Cancer, Gold Nanoparticle, Herceptin, Molecular Contrast, Optoacoustic Imaging, Sensitivity, Targeting",
author = "Mohammad Eghtedari and Massoud Motamedi and Vsevolod Popov and Kotov, {Nicholas A.} and Oraevsky, {Alexander A.}",
year = "2004",
doi = "10.1117/12.534464",
language = "English (US)",
volume = "5",
pages = "21--28",
editor = "A.A. Oraevsky and L.V. Wang",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
edition = "9",

}

TY - GEN

T1 - Optoacoustic imaging of gold nanoparticles targeted to breast cancer cells

AU - Eghtedari, Mohammad

AU - Motamedi, Massoud

AU - Popov, Vsevolod

AU - Kotov, Nicholas A.

AU - Oraevsky, Alexander A.

PY - 2004

Y1 - 2004

N2 - Optoacoustic Tomography (OAT) is a rapidly growing technology that enables noninvasive deep imaging of biological tissues based on their light absorption. In OAT, the interaction of a pulsed laser with tissue increases the temperature of the absorbing components in a confined volume of tissue. Rapid perturbation of the temperature (<1°C) deep within tissue produces weak acoustic waves that easily travel to the surface of the tissue with minor attenuation. Abnormal angiogenesis in a malignant tumor, that increases its blood content, makes a native contrast for optoacoustic imaging; however, the application of OAT for early detection of malignant tumors requires the enhancement of optoacoustic signals originated from tumor by using an exogenous contrast agent. Due to their strong absorption, we have used gold nanoparticles (NP) as a contrast agent. 40nm spherical gold nanoparticles were attached to monoclonal antibody to target cell surface of breast cancer cells. The targeted cancer cells were implanted at depth of 5-6cm within a gelatinous object that optically resembles human breast. Experimental sensitivity measurements along with theoretical analysis showed that our optoacoustic imaging system is capable of detecting a phantom breast tumor with the volume of 0.15ml, which is composed of 25 million NP-targeted cancer cells, at a depth of 5 centimeters in vitro.

AB - Optoacoustic Tomography (OAT) is a rapidly growing technology that enables noninvasive deep imaging of biological tissues based on their light absorption. In OAT, the interaction of a pulsed laser with tissue increases the temperature of the absorbing components in a confined volume of tissue. Rapid perturbation of the temperature (<1°C) deep within tissue produces weak acoustic waves that easily travel to the surface of the tissue with minor attenuation. Abnormal angiogenesis in a malignant tumor, that increases its blood content, makes a native contrast for optoacoustic imaging; however, the application of OAT for early detection of malignant tumors requires the enhancement of optoacoustic signals originated from tumor by using an exogenous contrast agent. Due to their strong absorption, we have used gold nanoparticles (NP) as a contrast agent. 40nm spherical gold nanoparticles were attached to monoclonal antibody to target cell surface of breast cancer cells. The targeted cancer cells were implanted at depth of 5-6cm within a gelatinous object that optically resembles human breast. Experimental sensitivity measurements along with theoretical analysis showed that our optoacoustic imaging system is capable of detecting a phantom breast tumor with the volume of 0.15ml, which is composed of 25 million NP-targeted cancer cells, at a depth of 5 centimeters in vitro.

KW - Cancer

KW - Gold Nanoparticle

KW - Herceptin

KW - Molecular Contrast

KW - Optoacoustic Imaging

KW - Sensitivity

KW - Targeting

UR - http://www.scopus.com/inward/record.url?scp=11144278223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144278223&partnerID=8YFLogxK

U2 - 10.1117/12.534464

DO - 10.1117/12.534464

M3 - Conference contribution

AN - SCOPUS:11144278223

VL - 5

SP - 21

EP - 28

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

A2 - Oraevsky, A.A.

A2 - Wang, L.V.

ER -