Optoacoustic system for 3D functional and molecular imaging in nude mice

Matthew P. Fronheiser, Alan Stein, Don Herzog, Scott Thompson, Anton Liopo, Mohammad Eghtedari, Massoud Motamedi, Sergey Ermilov, Andre Conjusteau, Reda Gharieb, Ron Lacewell, Tom Miller, Ketan Mehta, Alexander A. Oraevsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

A three-dimensional laser optoacoustic imaging system was developed, which combines the advantages of optical spectroscopy and high resolution ultrasonic detection, to produce high contrast maps of optical absorbance in tissues. This system was tested in a nude mouse model of breast cancer and produced tissue images of tumors and vasculature. The imaging can utilize either optical properties of hemoglobin and oxyhemoglobin, which are the main endogenous tissue chromophores in the red and near-infrared spectral ranges, or exogenous contrast agent based on gold nanorods. Visualization of tissue molecules targeted by the contrast agent provides molecular information. Visulization of blood at multiple colors of light provides functional information on blood concentration and oxygen saturation. Optoacoustic imaging, using two or more laser illumination wavelengths, permits an assessment of the angiogenesis-related microvasculature, and thereby, an evaluation of the tumor stage and its metastatic potential. The optoacoustic imaging system was also used to generate molecular images of the malignancy-related receptors induced by the xenografts of BT474 mammary adenocarcinoma cells in nude mice. The development of the latter images was facilitated by the use of an optoacoustic contrast agent that utilizes gold nanorods conjugated to monoclonal antibody raised against HER2/neu antigens. These nanorods possess a very strong optical absorption peak that can be tuned in the near-infrared by changing their aspect ratio. The effective conversion of the optical energy into heat by the gold nanorods, followed by the thermal expansion of the surrounding water, makes these nanoparticles an effective optoacoustic contrast agent. Optical scattering methods and x-ray tomography may also benefit from the application of this contrast agent. Administration of the gold nanorod bioconjugates to mice resulted in an enhanced contrast of breast tumors relative the background of normal tissues in the nude mouse model. The combination of this novel contrast agent and optoacoustic imaging has the potential to become a useful imaging modality, for preclinical research in murine models of cancer and other human diseases.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6856
DOIs
StatePublished - 2008
Event9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008 - San Jose, CA, United States
Duration: Jan 20 2008Jan 23 2008

Other

Other9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008
CountryUnited States
CitySan Jose, CA
Period1/20/081/23/08

Fingerprint

Molecular imaging
Photoacoustic effect
Nanorods
Tissue
Gold
Imaging techniques
Tumors
Imaging systems
Blood
Infrared radiation
Monoclonal antibodies
Lasers
Hemoglobin
Chromophores
Antigens
Light absorption
Thermal expansion
Tomography
Aspect ratio
Visualization

Keywords

  • Functional imaging
  • Molecular imaging
  • Optoacoustic imaging
  • Small animal imaging

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Fronheiser, M. P., Stein, A., Herzog, D., Thompson, S., Liopo, A., Eghtedari, M., ... Oraevsky, A. A. (2008). Optoacoustic system for 3D functional and molecular imaging in nude mice. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6856). [68560R] https://doi.org/10.1117/12.770461

Optoacoustic system for 3D functional and molecular imaging in nude mice. / Fronheiser, Matthew P.; Stein, Alan; Herzog, Don; Thompson, Scott; Liopo, Anton; Eghtedari, Mohammad; Motamedi, Massoud; Ermilov, Sergey; Conjusteau, Andre; Gharieb, Reda; Lacewell, Ron; Miller, Tom; Mehta, Ketan; Oraevsky, Alexander A.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6856 2008. 68560R.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fronheiser, MP, Stein, A, Herzog, D, Thompson, S, Liopo, A, Eghtedari, M, Motamedi, M, Ermilov, S, Conjusteau, A, Gharieb, R, Lacewell, R, Miller, T, Mehta, K & Oraevsky, AA 2008, Optoacoustic system for 3D functional and molecular imaging in nude mice. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6856, 68560R, 9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008, San Jose, CA, United States, 1/20/08. https://doi.org/10.1117/12.770461
Fronheiser MP, Stein A, Herzog D, Thompson S, Liopo A, Eghtedari M et al. Optoacoustic system for 3D functional and molecular imaging in nude mice. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6856. 2008. 68560R https://doi.org/10.1117/12.770461
Fronheiser, Matthew P. ; Stein, Alan ; Herzog, Don ; Thompson, Scott ; Liopo, Anton ; Eghtedari, Mohammad ; Motamedi, Massoud ; Ermilov, Sergey ; Conjusteau, Andre ; Gharieb, Reda ; Lacewell, Ron ; Miller, Tom ; Mehta, Ketan ; Oraevsky, Alexander A. / Optoacoustic system for 3D functional and molecular imaging in nude mice. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6856 2008.
@inproceedings{5dc4381e2a9b4be19007f44d7182d8f8,
title = "Optoacoustic system for 3D functional and molecular imaging in nude mice",
abstract = "A three-dimensional laser optoacoustic imaging system was developed, which combines the advantages of optical spectroscopy and high resolution ultrasonic detection, to produce high contrast maps of optical absorbance in tissues. This system was tested in a nude mouse model of breast cancer and produced tissue images of tumors and vasculature. The imaging can utilize either optical properties of hemoglobin and oxyhemoglobin, which are the main endogenous tissue chromophores in the red and near-infrared spectral ranges, or exogenous contrast agent based on gold nanorods. Visualization of tissue molecules targeted by the contrast agent provides molecular information. Visulization of blood at multiple colors of light provides functional information on blood concentration and oxygen saturation. Optoacoustic imaging, using two or more laser illumination wavelengths, permits an assessment of the angiogenesis-related microvasculature, and thereby, an evaluation of the tumor stage and its metastatic potential. The optoacoustic imaging system was also used to generate molecular images of the malignancy-related receptors induced by the xenografts of BT474 mammary adenocarcinoma cells in nude mice. The development of the latter images was facilitated by the use of an optoacoustic contrast agent that utilizes gold nanorods conjugated to monoclonal antibody raised against HER2/neu antigens. These nanorods possess a very strong optical absorption peak that can be tuned in the near-infrared by changing their aspect ratio. The effective conversion of the optical energy into heat by the gold nanorods, followed by the thermal expansion of the surrounding water, makes these nanoparticles an effective optoacoustic contrast agent. Optical scattering methods and x-ray tomography may also benefit from the application of this contrast agent. Administration of the gold nanorod bioconjugates to mice resulted in an enhanced contrast of breast tumors relative the background of normal tissues in the nude mouse model. The combination of this novel contrast agent and optoacoustic imaging has the potential to become a useful imaging modality, for preclinical research in murine models of cancer and other human diseases.",
keywords = "Functional imaging, Molecular imaging, Optoacoustic imaging, Small animal imaging",
author = "Fronheiser, {Matthew P.} and Alan Stein and Don Herzog and Scott Thompson and Anton Liopo and Mohammad Eghtedari and Massoud Motamedi and Sergey Ermilov and Andre Conjusteau and Reda Gharieb and Ron Lacewell and Tom Miller and Ketan Mehta and Oraevsky, {Alexander A.}",
year = "2008",
doi = "10.1117/12.770461",
language = "English (US)",
isbn = "9780819470317",
volume = "6856",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Optoacoustic system for 3D functional and molecular imaging in nude mice

AU - Fronheiser, Matthew P.

AU - Stein, Alan

AU - Herzog, Don

AU - Thompson, Scott

AU - Liopo, Anton

AU - Eghtedari, Mohammad

AU - Motamedi, Massoud

AU - Ermilov, Sergey

AU - Conjusteau, Andre

AU - Gharieb, Reda

AU - Lacewell, Ron

AU - Miller, Tom

AU - Mehta, Ketan

AU - Oraevsky, Alexander A.

PY - 2008

Y1 - 2008

N2 - A three-dimensional laser optoacoustic imaging system was developed, which combines the advantages of optical spectroscopy and high resolution ultrasonic detection, to produce high contrast maps of optical absorbance in tissues. This system was tested in a nude mouse model of breast cancer and produced tissue images of tumors and vasculature. The imaging can utilize either optical properties of hemoglobin and oxyhemoglobin, which are the main endogenous tissue chromophores in the red and near-infrared spectral ranges, or exogenous contrast agent based on gold nanorods. Visualization of tissue molecules targeted by the contrast agent provides molecular information. Visulization of blood at multiple colors of light provides functional information on blood concentration and oxygen saturation. Optoacoustic imaging, using two or more laser illumination wavelengths, permits an assessment of the angiogenesis-related microvasculature, and thereby, an evaluation of the tumor stage and its metastatic potential. The optoacoustic imaging system was also used to generate molecular images of the malignancy-related receptors induced by the xenografts of BT474 mammary adenocarcinoma cells in nude mice. The development of the latter images was facilitated by the use of an optoacoustic contrast agent that utilizes gold nanorods conjugated to monoclonal antibody raised against HER2/neu antigens. These nanorods possess a very strong optical absorption peak that can be tuned in the near-infrared by changing their aspect ratio. The effective conversion of the optical energy into heat by the gold nanorods, followed by the thermal expansion of the surrounding water, makes these nanoparticles an effective optoacoustic contrast agent. Optical scattering methods and x-ray tomography may also benefit from the application of this contrast agent. Administration of the gold nanorod bioconjugates to mice resulted in an enhanced contrast of breast tumors relative the background of normal tissues in the nude mouse model. The combination of this novel contrast agent and optoacoustic imaging has the potential to become a useful imaging modality, for preclinical research in murine models of cancer and other human diseases.

AB - A three-dimensional laser optoacoustic imaging system was developed, which combines the advantages of optical spectroscopy and high resolution ultrasonic detection, to produce high contrast maps of optical absorbance in tissues. This system was tested in a nude mouse model of breast cancer and produced tissue images of tumors and vasculature. The imaging can utilize either optical properties of hemoglobin and oxyhemoglobin, which are the main endogenous tissue chromophores in the red and near-infrared spectral ranges, or exogenous contrast agent based on gold nanorods. Visualization of tissue molecules targeted by the contrast agent provides molecular information. Visulization of blood at multiple colors of light provides functional information on blood concentration and oxygen saturation. Optoacoustic imaging, using two or more laser illumination wavelengths, permits an assessment of the angiogenesis-related microvasculature, and thereby, an evaluation of the tumor stage and its metastatic potential. The optoacoustic imaging system was also used to generate molecular images of the malignancy-related receptors induced by the xenografts of BT474 mammary adenocarcinoma cells in nude mice. The development of the latter images was facilitated by the use of an optoacoustic contrast agent that utilizes gold nanorods conjugated to monoclonal antibody raised against HER2/neu antigens. These nanorods possess a very strong optical absorption peak that can be tuned in the near-infrared by changing their aspect ratio. The effective conversion of the optical energy into heat by the gold nanorods, followed by the thermal expansion of the surrounding water, makes these nanoparticles an effective optoacoustic contrast agent. Optical scattering methods and x-ray tomography may also benefit from the application of this contrast agent. Administration of the gold nanorod bioconjugates to mice resulted in an enhanced contrast of breast tumors relative the background of normal tissues in the nude mouse model. The combination of this novel contrast agent and optoacoustic imaging has the potential to become a useful imaging modality, for preclinical research in murine models of cancer and other human diseases.

KW - Functional imaging

KW - Molecular imaging

KW - Optoacoustic imaging

KW - Small animal imaging

UR - http://www.scopus.com/inward/record.url?scp=42149095363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42149095363&partnerID=8YFLogxK

U2 - 10.1117/12.770461

DO - 10.1117/12.770461

M3 - Conference contribution

SN - 9780819470317

VL - 6856

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -