Oral and Conjunctival Exposure of Nonhuman Primates to Low Doses of Ebola Makona Virus

Chad E. Mire; Joan B. Geisbert; Krystle N. Agans; Daniel J. Deer; Karla A. Fenton; Thomas W. Geisbert

doi:10.1093/infdis/jiw149

Oral and Conjunctival Exposure of Nonhuman Primates to Low Doses of Ebola Makona Virus

Chad E. Mire, Joan B. Geisbert, Krystle N. Agans, Daniel J. Deer, Karla A. Fenton, Thomas W. Geisbert

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Nonhuman primate (NHP) models of Ebola virus (EBOV) infection primarily use parenteral or aerosol routes of exposure. Uniform lethality can be achieved in these models at low doses of EBOV (≤100 plaque-forming units [PFU]). Here, we exposed NHPs to low doses of EBOV (Makona strain) by the oral or conjunctival routes. Surprisingly, animals exposed to 10 PFU by either route showed no signs of disease. Exposure to 100 PFU resulted in illness and/or lethal infection. These results suggest that these more natural routes require higher doses of EBOV to produce disease or that there may be differences between Makona and historical strains.

Original language	English (US)
Pages (from-to)	S263-S267
Journal	Journal of Infectious Diseases
Volume	214
DOIs	https://doi.org/10.1093/infdis/jiw149
State	Published - Oct 15 2016

Keywords

Ebola virus
Nonhuman primate
Pathogenesis

ASJC Scopus subject areas

General Medicine

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10.1093/infdis/jiw149

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title = "Oral and Conjunctival Exposure of Nonhuman Primates to Low Doses of Ebola Makona Virus",

abstract = "Nonhuman primate (NHP) models of Ebola virus (EBOV) infection primarily use parenteral or aerosol routes of exposure. Uniform lethality can be achieved in these models at low doses of EBOV (≤100 plaque-forming units [PFU]). Here, we exposed NHPs to low doses of EBOV (Makona strain) by the oral or conjunctival routes. Surprisingly, animals exposed to 10 PFU by either route showed no signs of disease. Exposure to 100 PFU resulted in illness and/or lethal infection. These results suggest that these more natural routes require higher doses of EBOV to produce disease or that there may be differences between Makona and historical strains.",

keywords = "Ebola virus, Nonhuman primate, Pathogenesis",

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T1 - Oral and Conjunctival Exposure of Nonhuman Primates to Low Doses of Ebola Makona Virus

AU - Mire, Chad E.

AU - Geisbert, Joan B.

AU - Agans, Krystle N.

AU - Deer, Daniel J.

AU - Fenton, Karla A.

AU - Geisbert, Thomas W.

PY - 2016/10/15

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AB - Nonhuman primate (NHP) models of Ebola virus (EBOV) infection primarily use parenteral or aerosol routes of exposure. Uniform lethality can be achieved in these models at low doses of EBOV (≤100 plaque-forming units [PFU]). Here, we exposed NHPs to low doses of EBOV (Makona strain) by the oral or conjunctival routes. Surprisingly, animals exposed to 10 PFU by either route showed no signs of disease. Exposure to 100 PFU resulted in illness and/or lethal infection. These results suggest that these more natural routes require higher doses of EBOV to produce disease or that there may be differences between Makona and historical strains.

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KW - Nonhuman primate

KW - Pathogenesis

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Oral and Conjunctival Exposure of Nonhuman Primates to Low Doses of Ebola Makona Virus

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Keywords

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