Oral glutamine reduces bacterial translocation following abdominal radiation

Wiley W. Souba, Vicki Klimberg, R. Dean Hautamaki, William H. Mendenhall, Frank C. Bova, Richard J. Howard, Kirby I. Bland, Edward M. Copeland

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

The effect of dietary glutamine on bacterial translocation was studied in rats following administration of a single dose of abdominal radiation (1000 rad) that causes a reproducible mucosal injury and results in a high incidence of culture-positive mesenteric lymph nodes after radiation (XRT). Following XRT, rats received only the amino acid glutamine (3%, +GLN) in their drinking water or a control nonessential amino acid (glycine, -GLN). Diets were isonitrogenous and isovolumetric. Four days after XRT, rats were anesthetized and a laparotomy was performed. Mesenteric lymph nodes were sterilely excised and cultured. Arterial blood was also obtained for whole blood glutamine determination. Control rats received no XRT but received identical diets. In XRT rats who received the GLN-free diet, the incidence of culture positive mesenteric lymph nodes was 89% (eight of nine rats) while in the radiated rats receiving the GLN-enriched diet, the incidence fell to 20% (P < 0.05). In non-radiated control rats receiving GLN-enriched and GLN-depleted diets for 4 days, bacterial translocation occurred in zero of eight and one of eight rats, respectively (NS). Provision of glutamine to XRT rats resulted in higher blood levels of glutamine (408 ± 25 μM in XRT +GLN vs 311 ± 19 μM in XRT -GLN, P < 0.05). In addition, provision of GLN maintained mucosal mass and reduced weight loss (P < 0.05). The data lend further support to the hypothesis that glutamine helps maintain the gut mucosal barrier and thereby decreases the incidence of bacterial translocation following bowel injury. The possible mechanisms by which glutamine may reduce the incidence of culture-positive mesenteric lymph nodes following abdominal XRT are discussed.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of Surgical Research
Volume48
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

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Bacterial Translocation
Glutamine
Radiation
Diet
Lymph Nodes
Incidence
Amino Acids
Wounds and Injuries
Drinking Water
Glycine
Laparotomy
Weight Loss

ASJC Scopus subject areas

  • Surgery

Cite this

Souba, W. W., Klimberg, V., Hautamaki, R. D., Mendenhall, W. H., Bova, F. C., Howard, R. J., ... Copeland, E. M. (1990). Oral glutamine reduces bacterial translocation following abdominal radiation. Journal of Surgical Research, 48(1), 1-5. https://doi.org/10.1016/0022-4804(90)90136-P

Oral glutamine reduces bacterial translocation following abdominal radiation. / Souba, Wiley W.; Klimberg, Vicki; Hautamaki, R. Dean; Mendenhall, William H.; Bova, Frank C.; Howard, Richard J.; Bland, Kirby I.; Copeland, Edward M.

In: Journal of Surgical Research, Vol. 48, No. 1, 1990, p. 1-5.

Research output: Contribution to journalArticle

Souba, WW, Klimberg, V, Hautamaki, RD, Mendenhall, WH, Bova, FC, Howard, RJ, Bland, KI & Copeland, EM 1990, 'Oral glutamine reduces bacterial translocation following abdominal radiation', Journal of Surgical Research, vol. 48, no. 1, pp. 1-5. https://doi.org/10.1016/0022-4804(90)90136-P
Souba, Wiley W. ; Klimberg, Vicki ; Hautamaki, R. Dean ; Mendenhall, William H. ; Bova, Frank C. ; Howard, Richard J. ; Bland, Kirby I. ; Copeland, Edward M. / Oral glutamine reduces bacterial translocation following abdominal radiation. In: Journal of Surgical Research. 1990 ; Vol. 48, No. 1. pp. 1-5.
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abstract = "The effect of dietary glutamine on bacterial translocation was studied in rats following administration of a single dose of abdominal radiation (1000 rad) that causes a reproducible mucosal injury and results in a high incidence of culture-positive mesenteric lymph nodes after radiation (XRT). Following XRT, rats received only the amino acid glutamine (3{\%}, +GLN) in their drinking water or a control nonessential amino acid (glycine, -GLN). Diets were isonitrogenous and isovolumetric. Four days after XRT, rats were anesthetized and a laparotomy was performed. Mesenteric lymph nodes were sterilely excised and cultured. Arterial blood was also obtained for whole blood glutamine determination. Control rats received no XRT but received identical diets. In XRT rats who received the GLN-free diet, the incidence of culture positive mesenteric lymph nodes was 89{\%} (eight of nine rats) while in the radiated rats receiving the GLN-enriched diet, the incidence fell to 20{\%} (P < 0.05). In non-radiated control rats receiving GLN-enriched and GLN-depleted diets for 4 days, bacterial translocation occurred in zero of eight and one of eight rats, respectively (NS). Provision of glutamine to XRT rats resulted in higher blood levels of glutamine (408 ± 25 μM in XRT +GLN vs 311 ± 19 μM in XRT -GLN, P < 0.05). In addition, provision of GLN maintained mucosal mass and reduced weight loss (P < 0.05). The data lend further support to the hypothesis that glutamine helps maintain the gut mucosal barrier and thereby decreases the incidence of bacterial translocation following bowel injury. The possible mechanisms by which glutamine may reduce the incidence of culture-positive mesenteric lymph nodes following abdominal XRT are discussed.",
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