Ordered arrays of Ca2+-ATPase on the cytoplasmic surface of isolated sarcoplasmic reticulum

D. G. Ferguson, C. Franzini-Armstrong, L. Castellani, P. M. Hardwicke, Linda Kenney

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Isolated sarcoplasmic reticulum (SR) vesicles with polymerized calcium pump protein were freeze-dried and rotary shadowed following uranyl acetate stabilization. This technique allows direct observation of a single side of the vesicle without requiring optical filtering. The heads of individual ATPase molecules, projecting above the cytoplasmic surface, are clearly resolved in the replicas. Ca ATPase molecules form extensive arrays in vanadate-treated, rabbit SR vesicles and in gently isolated, native SR vesicles from scallop. Gentle isolation results in limited areas of orderly structure in native SR isolated from vertebrate muscles. Special attention is given to the effect of various shadow thicknesses on the appearance of the heads. This information is essential to the interpretation of images in the accompanying paper (Franzini-Armstrong, C., and D.J. Ferguson, 1985, Biophys. J., 48:607–615).

Original languageEnglish (US)
Pages (from-to)597-605
Number of pages9
JournalBiophysical Journal
Volume48
Issue number4
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

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Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Adenosine Triphosphatases
Pectinidae
Vanadates
Vertebrates
Head
Observation
Rabbits
Calcium
Muscles
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Ordered arrays of Ca2+-ATPase on the cytoplasmic surface of isolated sarcoplasmic reticulum. / Ferguson, D. G.; Franzini-Armstrong, C.; Castellani, L.; Hardwicke, P. M.; Kenney, Linda.

In: Biophysical Journal, Vol. 48, No. 4, 01.01.1985, p. 597-605.

Research output: Contribution to journalArticle

Ferguson, D. G. ; Franzini-Armstrong, C. ; Castellani, L. ; Hardwicke, P. M. ; Kenney, Linda. / Ordered arrays of Ca2+-ATPase on the cytoplasmic surface of isolated sarcoplasmic reticulum. In: Biophysical Journal. 1985 ; Vol. 48, No. 4. pp. 597-605.
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