Organ culture of amniochorionic membrane in vitro

S. J. Fortunato, Ramkumar Menon, K. F. Swan, T. W. Lyden

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

PROBLEM: The purpose of the study was to develop a novel method of amniochorionic membrane culture aimed at maintaining tissue integrity. METHOD: Amniochorionic membranes were collected from women prior to labor, undergoing elective cesarean section, with no history of infection or pregnancy related complication. Fetal membranes were maintained in culture for up to ten days. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 'house keeping' gene and inflammatory cytokine mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) at various periods of culture. mRNA localization was performed by in situ hybridization. RESULTS: GAPDH gene expression was seen throughout the culture period. Inflammatory cytokine mRNA (IL-1α, IL-1β and IL-6) were also detected during culture. Cellular and tissue morphology appeared normal. CONCLUSIONS: The culture technique we propose is a simple organ explant system which maintains the morphology and autocrine/paracrine relationships within this tissue.

Original languageEnglish (US)
Pages (from-to)184-187
Number of pages4
JournalAmerican Journal of Reproductive Immunology
Volume32
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Organ Culture Techniques
Glyceraldehyde-3-Phosphate Dehydrogenases
Interleukin-1
Messenger RNA
Membranes
Cytokines
Extraembryonic Membranes
Culture Techniques
Pregnancy Complications
Essential Genes
Reverse Transcriptase Polymerase Chain Reaction
Cesarean Section
In Situ Hybridization
Interleukin-6
Gene Expression
Infection
In Vitro Techniques

Keywords

  • Amniochorion
  • Cytokine
  • IL-1
  • IL-6
  • Organ culture
  • Pregnancy

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Fortunato, S. J., Menon, R., Swan, K. F., & Lyden, T. W. (1994). Organ culture of amniochorionic membrane in vitro. American Journal of Reproductive Immunology, 32(3), 184-187.

Organ culture of amniochorionic membrane in vitro. / Fortunato, S. J.; Menon, Ramkumar; Swan, K. F.; Lyden, T. W.

In: American Journal of Reproductive Immunology, Vol. 32, No. 3, 1994, p. 184-187.

Research output: Contribution to journalArticle

Fortunato, SJ, Menon, R, Swan, KF & Lyden, TW 1994, 'Organ culture of amniochorionic membrane in vitro', American Journal of Reproductive Immunology, vol. 32, no. 3, pp. 184-187.
Fortunato, S. J. ; Menon, Ramkumar ; Swan, K. F. ; Lyden, T. W. / Organ culture of amniochorionic membrane in vitro. In: American Journal of Reproductive Immunology. 1994 ; Vol. 32, No. 3. pp. 184-187.
@article{3db88f928a72445db9cecfd36a322286,
title = "Organ culture of amniochorionic membrane in vitro",
abstract = "PROBLEM: The purpose of the study was to develop a novel method of amniochorionic membrane culture aimed at maintaining tissue integrity. METHOD: Amniochorionic membranes were collected from women prior to labor, undergoing elective cesarean section, with no history of infection or pregnancy related complication. Fetal membranes were maintained in culture for up to ten days. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 'house keeping' gene and inflammatory cytokine mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) at various periods of culture. mRNA localization was performed by in situ hybridization. RESULTS: GAPDH gene expression was seen throughout the culture period. Inflammatory cytokine mRNA (IL-1α, IL-1β and IL-6) were also detected during culture. Cellular and tissue morphology appeared normal. CONCLUSIONS: The culture technique we propose is a simple organ explant system which maintains the morphology and autocrine/paracrine relationships within this tissue.",
keywords = "Amniochorion, Cytokine, IL-1, IL-6, Organ culture, Pregnancy",
author = "Fortunato, {S. J.} and Ramkumar Menon and Swan, {K. F.} and Lyden, {T. W.}",
year = "1994",
language = "English (US)",
volume = "32",
pages = "184--187",
journal = "American Journal of Reproductive Immunology and Microbiology",
issn = "1046-7408",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Organ culture of amniochorionic membrane in vitro

AU - Fortunato, S. J.

AU - Menon, Ramkumar

AU - Swan, K. F.

AU - Lyden, T. W.

PY - 1994

Y1 - 1994

N2 - PROBLEM: The purpose of the study was to develop a novel method of amniochorionic membrane culture aimed at maintaining tissue integrity. METHOD: Amniochorionic membranes were collected from women prior to labor, undergoing elective cesarean section, with no history of infection or pregnancy related complication. Fetal membranes were maintained in culture for up to ten days. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 'house keeping' gene and inflammatory cytokine mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) at various periods of culture. mRNA localization was performed by in situ hybridization. RESULTS: GAPDH gene expression was seen throughout the culture period. Inflammatory cytokine mRNA (IL-1α, IL-1β and IL-6) were also detected during culture. Cellular and tissue morphology appeared normal. CONCLUSIONS: The culture technique we propose is a simple organ explant system which maintains the morphology and autocrine/paracrine relationships within this tissue.

AB - PROBLEM: The purpose of the study was to develop a novel method of amniochorionic membrane culture aimed at maintaining tissue integrity. METHOD: Amniochorionic membranes were collected from women prior to labor, undergoing elective cesarean section, with no history of infection or pregnancy related complication. Fetal membranes were maintained in culture for up to ten days. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 'house keeping' gene and inflammatory cytokine mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) at various periods of culture. mRNA localization was performed by in situ hybridization. RESULTS: GAPDH gene expression was seen throughout the culture period. Inflammatory cytokine mRNA (IL-1α, IL-1β and IL-6) were also detected during culture. Cellular and tissue morphology appeared normal. CONCLUSIONS: The culture technique we propose is a simple organ explant system which maintains the morphology and autocrine/paracrine relationships within this tissue.

KW - Amniochorion

KW - Cytokine

KW - IL-1

KW - IL-6

KW - Organ culture

KW - Pregnancy

UR - http://www.scopus.com/inward/record.url?scp=0028670109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028670109&partnerID=8YFLogxK

M3 - Article

C2 - 7880402

AN - SCOPUS:0028670109

VL - 32

SP - 184

EP - 187

JO - American Journal of Reproductive Immunology and Microbiology

JF - American Journal of Reproductive Immunology and Microbiology

SN - 1046-7408

IS - 3

ER -