Organ culture of amniochorionic membrane in vitro

S. J. Fortunato, R. Menon, K. F. Swan, T. W. Lyden

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

PROBLEM: The purpose of the study was to develop a novel method of amniochorionic membrane culture aimed at maintaining tissue integrity. METHOD: Amniochorionic membranes were collected from women prior to labor, undergoing elective cesarean section, with no history of infection or pregnancy related complication. Fetal membranes were maintained in culture for up to ten days. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 'house keeping' gene and inflammatory cytokine mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) at various periods of culture. mRNA localization was performed by in situ hybridization. RESULTS: GAPDH gene expression was seen throughout the culture period. Inflammatory cytokine mRNA (IL-1α, IL-1β and IL-6) were also detected during culture. Cellular and tissue morphology appeared normal. CONCLUSIONS: The culture technique we propose is a simple organ explant system which maintains the morphology and autocrine/paracrine relationships within this tissue.

Original languageEnglish (US)
Pages (from-to)184-187
Number of pages4
JournalAmerican Journal of Reproductive Immunology
Volume32
Issue number3
StatePublished - Dec 1 1994
Externally publishedYes

Keywords

  • Amniochorion
  • Cytokine
  • IL-1
  • IL-6
  • Organ culture
  • Pregnancy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

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  • Cite this

    Fortunato, S. J., Menon, R., Swan, K. F., & Lyden, T. W. (1994). Organ culture of amniochorionic membrane in vitro. American Journal of Reproductive Immunology, 32(3), 184-187.