Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon

Ronald D. Vale, Bruce J. Schnapp, Thomas S. Reese, Michael Sheetz

Research output: Contribution to journalArticle

215 Citations (Scopus)

Abstract

A reconstituted system for examining directed organelle movements along purified microtubules has been developed. Axoplasm from the squid giant axon was separated into soluble supernatant and organelle-enriched fractions. Movement of axoplasmic organelles along MAP-free microtubules occurred consistently only after addition of axoplasmic supernatant and ATP. The velocity of such organelle movement (1.6μ/sec) was the same as in dissociated axoplasm. The axoplasmic supernatant also supported movement of microtubules along a glass surface and movement of carboxylated latex beads along microtubules at 0.5 μ/sec. The direction of microtubule movement on glass was opposite to that of organelle and bead movement on microtubules. The factors supporting movements of microtubules, beads, and organelles were sensitive to heat, trypsin, AMP-PNP and 100 μM vanadate. All of these movements may be driven by a single, soluble ATPase that binds reversibly to organelles, beads, or glass and generates a translocating force on a microtubule.

Original languageEnglish (US)
Pages (from-to)559-569
Number of pages11
JournalCell
Volume40
Issue number3
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Decapodiformes
Microtubules
Organelles
Axons
Glass
Adenylyl Imidodiphosphate
Vanadates
Latex
Trypsin
Adenosine Triphosphatases
Adenosine Triphosphate
Microspheres
Hot Temperature

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon. / Vale, Ronald D.; Schnapp, Bruce J.; Reese, Thomas S.; Sheetz, Michael.

In: Cell, Vol. 40, No. 3, 01.01.1985, p. 559-569.

Research output: Contribution to journalArticle

Vale, Ronald D. ; Schnapp, Bruce J. ; Reese, Thomas S. ; Sheetz, Michael. / Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon. In: Cell. 1985 ; Vol. 40, No. 3. pp. 559-569.
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