Ornithine decarboxylase activity in cerebral post-ischemic reperfusion damage

Effect of methionine sulfoximine

C. Di Giacomo, V. Sorrenti, R. Acquaviva, A. Campisi, G. Vanella, J. R. Perez-Polo, A. Vanella

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Excessive activation of glutamate receptors via the N-methyl-D-aspartate (NMDA) subtype appears to play a role in the sequence of cellular events which lead to irreversible ischemic damage to neurons. Furthermore, NMDA receptor activation induces a stimulation of omithine decarboxylase (ODC), the rate-limiting enzyme for polyamine (PA) biosynthesis. In order to better understand the role of PA we have measured ODC activity and the effect of methionine sulfoximine (MSO), a molecule able to stimulate ODC, on a model of transient cerebral ischemia. There was a significant increase in ODC activity in the rat cerebral cortex during post-ischemic reperfusion. The treatment with MSO induced a significant decrease in cerebral glutamine synthetase activity accompanied by a marked increase in ODC activity. In MSO-pretreated rats there was a significant decrease in the survival rate when compared to untreated ischemic rats.

Original languageEnglish (US)
Pages (from-to)1145-1150
Number of pages6
JournalNeurochemical Research
Volume22
Issue number9
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Methionine Sulfoximine
Ornithine Decarboxylase
Carboxy-Lyases
Reperfusion Injury
Rats
Polyamines
Chemical activation
Glutamate-Ammonia Ligase
Biosynthesis
Transient Ischemic Attack
Glutamate Receptors
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Cerebral Cortex
Neurons
Reperfusion
Molecules
Enzymes

Keywords

  • Cerebral ischemia
  • GS
  • ODC
  • Polyamines
  • Reperfusion
  • Survival

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Di Giacomo, C., Sorrenti, V., Acquaviva, R., Campisi, A., Vanella, G., Perez-Polo, J. R., & Vanella, A. (1997). Ornithine decarboxylase activity in cerebral post-ischemic reperfusion damage: Effect of methionine sulfoximine. Neurochemical Research, 22(9), 1145-1150. https://doi.org/10.1023/A:1027321420075

Ornithine decarboxylase activity in cerebral post-ischemic reperfusion damage : Effect of methionine sulfoximine. / Di Giacomo, C.; Sorrenti, V.; Acquaviva, R.; Campisi, A.; Vanella, G.; Perez-Polo, J. R.; Vanella, A.

In: Neurochemical Research, Vol. 22, No. 9, 1997, p. 1145-1150.

Research output: Contribution to journalArticle

Di Giacomo, C, Sorrenti, V, Acquaviva, R, Campisi, A, Vanella, G, Perez-Polo, JR & Vanella, A 1997, 'Ornithine decarboxylase activity in cerebral post-ischemic reperfusion damage: Effect of methionine sulfoximine', Neurochemical Research, vol. 22, no. 9, pp. 1145-1150. https://doi.org/10.1023/A:1027321420075
Di Giacomo, C. ; Sorrenti, V. ; Acquaviva, R. ; Campisi, A. ; Vanella, G. ; Perez-Polo, J. R. ; Vanella, A. / Ornithine decarboxylase activity in cerebral post-ischemic reperfusion damage : Effect of methionine sulfoximine. In: Neurochemical Research. 1997 ; Vol. 22, No. 9. pp. 1145-1150.
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