Ornithine decarboxylase activity in cerebral post-ischemic reperfusion damage: Effect of methionine sulfoximine

C. Di Giacomo, V. Sorrenti, R. Acquaviva, A. Campisi, G. Vanella, J. R. Perez-Polo, A. Vanella

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Excessive activation of glutamate receptors via the N-methyl-D-aspartate (NMDA) subtype appears to play a role in the sequence of cellular events which lead to irreversible ischemic damage to neurons. Furthermore, NMDA receptor activation induces a stimulation of omithine decarboxylase (ODC), the rate-limiting enzyme for polyamine (PA) biosynthesis. In order to better understand the role of PA we have measured ODC activity and the effect of methionine sulfoximine (MSO), a molecule able to stimulate ODC, on a model of transient cerebral ischemia. There was a significant increase in ODC activity in the rat cerebral cortex during post-ischemic reperfusion. The treatment with MSO induced a significant decrease in cerebral glutamine synthetase activity accompanied by a marked increase in ODC activity. In MSO-pretreated rats there was a significant decrease in the survival rate when compared to untreated ischemic rats.

Original languageEnglish (US)
Pages (from-to)1145-1150
Number of pages6
JournalNeurochemical Research
Volume22
Issue number9
DOIs
StatePublished - 1997
Externally publishedYes

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Keywords

  • Cerebral ischemia
  • GS
  • ODC
  • Polyamines
  • Reperfusion
  • Survival

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Di Giacomo, C., Sorrenti, V., Acquaviva, R., Campisi, A., Vanella, G., Perez-Polo, J. R., & Vanella, A. (1997). Ornithine decarboxylase activity in cerebral post-ischemic reperfusion damage: Effect of methionine sulfoximine. Neurochemical Research, 22(9), 1145-1150. https://doi.org/10.1023/A:1027321420075