TY - JOUR
T1 - Oropouche orthobunyavirus
T2 - Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
AU - Nunes, Márcio Roberto Teixeira
AU - de Souza, William Marciel
AU - Savji, Nazir
AU - Figueiredo, Mário Luís
AU - Cardoso, Jedson Ferreira
AU - da Silva, Sandro Patroca
AU - da Silva de Lima, Clayton Pereira
AU - Vasconcelos, Helena Baldez
AU - Rodrigues, Sueli Guerreiro
AU - Ian Lipkin, W.
AU - Vasconcelos, Pedro Fernando Costa
AU - Palacios, Gustavo
N1 - Publisher Copyright:
© 2018
PY - 2019/3
Y1 - 2019/3
N2 - Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Pará and Maranhão states, nine as genotype II from Pará and Rondônia states confirmed, four classified into genotype III from Acre, Maranhão, Minas Gerais and Rondônia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.
AB - Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Pará and Maranhão states, nine as genotype II from Pará and Rondônia states confirmed, four classified into genotype III from Acre, Maranhão, Minas Gerais and Rondônia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.
KW - Arbovirus
KW - Molecular diagnosis
KW - Oropouche orthobunyavirus
KW - Peribunyaviridae
KW - Viral genotyping
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U2 - 10.1016/j.meegid.2018.11.020
DO - 10.1016/j.meegid.2018.11.020
M3 - Article
C2 - 30504003
AN - SCOPUS:85057864280
SN - 1567-1348
VL - 68
SP - 16
EP - 22
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -