Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

Márcio Roberto Teixeira Nunes, William Marciel de Souza, Nazir Savji, Mário Luís Figueiredo, Jedson Ferreira Cardoso, Sandro Patroca da Silva, Clayton Pereira da Silva de Lima, Helena Baldez Vasconcelos, Sueli Guerreiro Rodrigues, W. Ian Lipkin, Pedro Fernando Costa Vasconcelos, Gustavo Palacios

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Pará and Maranhão states, nine as genotype II from Pará and Rondônia states confirmed, four classified into genotype III from Acre, Maranhão, Minas Gerais and Rondônia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.

Original languageEnglish (US)
Pages (from-to)16-22
Number of pages7
JournalInfection, Genetics and Evolution
Volume68
DOIs
StatePublished - Mar 2019
Externally publishedYes

Keywords

  • Arbovirus
  • Molecular diagnosis
  • Oropouche orthobunyavirus
  • Peribunyaviridae
  • Viral genotyping

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments'. Together they form a unique fingerprint.

Cite this