Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

Márcio Roberto Teixeira Nunes; William Marciel de Souza; Nazir Savji; Mário Luís Figueiredo; Jedson Ferreira Cardoso; Sandro Patroca da Silva; Clayton Pereira da Silva de Lima; Helena Baldez Vasconcelos; Sueli Guerreiro Rodrigues; W. Ian Lipkin; Pedro Fernando Costa Vasconcelos; Gustavo Palacios

doi:10.1016/j.meegid.2018.11.020

Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

Márcio Roberto Teixeira Nunes
, William Marciel de Souza
, Nazir Savji
, Mário Luís Figueiredo
, Jedson Ferreira Cardoso
, Sandro Patroca da Silva
, Clayton Pereira da Silva de Lima
, Helena Baldez Vasconcelos
, Sueli Guerreiro Rodrigues
, W. Ian Lipkin
, Pedro Fernando Costa Vasconcelos
, Gustavo Palacios

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Pará and Maranhão states, nine as genotype II from Pará and Rondônia states confirmed, four classified into genotype III from Acre, Maranhão, Minas Gerais and Rondônia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.

Original language	English (US)
Pages (from-to)	16-22
Number of pages	7
Journal	Infection, Genetics and Evolution
Volume	68
DOIs	https://doi.org/10.1016/j.meegid.2018.11.020
State	Published - Mar 2019
Externally published	Yes

Keywords

Arbovirus
Molecular diagnosis
Oropouche orthobunyavirus
Peribunyaviridae
Viral genotyping

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Microbiology (medical)
Infectious Diseases

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10.1016/j.meegid.2018.11.020

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Nunes, M. R. T., de Souza, W. M., Savji, N., Figueiredo, M. L., Cardoso, J. F., da Silva, S. P., da Silva de Lima, C. P., Vasconcelos, H. B., Rodrigues, S. G., Ian Lipkin, W., Vasconcelos, P. F. C., & Palacios, G. (2019). Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments. Infection, Genetics and Evolution, 68, 16-22. https://doi.org/10.1016/j.meegid.2018.11.020

Nunes, MRT, de Souza, WM, Savji, N, Figueiredo, ML, Cardoso, JF, da Silva, SP, da Silva de Lima, CP, Vasconcelos, HB, Rodrigues, SG, Ian Lipkin, W, Vasconcelos, PFC & Palacios, G 2019, 'Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments', Infection, Genetics and Evolution, vol. 68, pp. 16-22. https://doi.org/10.1016/j.meegid.2018.11.020

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title = "Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments",

abstract = "Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Par{\'a} and Maranh{\~a}o states, nine as genotype II from Par{\'a} and Rond{\^o}nia states confirmed, four classified into genotype III from Acre, Maranh{\~a}o, Minas Gerais and Rond{\^o}nia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.",

keywords = "Arbovirus, Molecular diagnosis, Oropouche orthobunyavirus, Peribunyaviridae, Viral genotyping",

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year = "2019",

month = mar,

doi = "10.1016/j.meegid.2018.11.020",

language = "English (US)",

volume = "68",

pages = "16--22",

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AU - Nunes, Márcio Roberto Teixeira

AU - de Souza, William Marciel

AU - Savji, Nazir

AU - Figueiredo, Mário Luís

AU - Cardoso, Jedson Ferreira

AU - da Silva, Sandro Patroca

AU - da Silva de Lima, Clayton Pereira

AU - Vasconcelos, Helena Baldez

AU - Rodrigues, Sueli Guerreiro

AU - Ian Lipkin, W.

AU - Vasconcelos, Pedro Fernando Costa

AU - Palacios, Gustavo

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Y1 - 2019/3

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AB - Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Pará and Maranhão states, nine as genotype II from Pará and Rondônia states confirmed, four classified into genotype III from Acre, Maranhão, Minas Gerais and Rondônia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.

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KW - Molecular diagnosis

KW - Oropouche orthobunyavirus

KW - Peribunyaviridae

KW - Viral genotyping

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JF - Infection, Genetics and Evolution

ER -

Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

Abstract

Keywords

ASJC Scopus subject areas

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