Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells

Varisa Pongrakhananon, Preedakorn Chunhacha, Pithi Chanvorachote

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The migratory capability of cancer cells is one of the most important hallmarks reflecting metastatic potential. Ouabain, an endogenous cardiac glycoside produced by the adrenal gland, has been previously reported to have anti-tumor activities; however, its role in the regulation of cancer cell migration remains unknown. The present study has revealed that treatment with ouabain at physiological concentrations is able to inhibit the migratory activities of human lung cancer H292 cells. The negative effects of ouabain were found to be mediated through the suppression of migration regulatory proteins, such as focal adhesion kinase (FAK), ATP-dependent tyrosine kinase (Akt), and cell division cycle 42 (Cdc42). We found that the observed actions of ouabain were mediated via a reactive oxygen species (ROS)-dependent mechanism because the addition of ROS scavengers (N-acetylcysteine and glutathione) could reverse the effect of ouabain on cell migration. Furthermore, ouabain was shown to inhibit the spheroidal tumor growth and decrease the cancer cell adhesion to endothelial cells. However, the compound had no significant effect on anoikis of the cells. Together, these findings shed light on the understanding of cancer cell biology by exploring the novel function of this endogenous human substance.

Original languageEnglish (US)
Article numbere68623
JournalPLoS One
Volume8
Issue number7
DOIs
StatePublished - Jul 10 2013
Externally publishedYes

Fingerprint

ouabain
Ouabain
lung neoplasms
migratory behavior
Lung Neoplasms
Cells
Neoplasms
cell movement
Cell Movement
Tumors
reactive oxygen species
Reactive Oxygen Species
non-specific protein-tyrosine kinase
Anoikis
Cytology
cardiac glycosides
Cardiac Glycosides
Focal Adhesion Protein-Tyrosine Kinases
acetylcysteine
neoplasms

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pongrakhananon, V., Chunhacha, P., & Chanvorachote, P. (2013). Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells. PLoS One, 8(7), [e68623]. https://doi.org/10.1371/journal.pone.0068623

Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells. / Pongrakhananon, Varisa; Chunhacha, Preedakorn; Chanvorachote, Pithi.

In: PLoS One, Vol. 8, No. 7, e68623, 10.07.2013.

Research output: Contribution to journalArticle

Pongrakhananon, V, Chunhacha, P & Chanvorachote, P 2013, 'Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells', PLoS One, vol. 8, no. 7, e68623. https://doi.org/10.1371/journal.pone.0068623
Pongrakhananon V, Chunhacha P, Chanvorachote P. Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells. PLoS One. 2013 Jul 10;8(7). e68623. https://doi.org/10.1371/journal.pone.0068623
Pongrakhananon, Varisa ; Chunhacha, Preedakorn ; Chanvorachote, Pithi. / Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells. In: PLoS One. 2013 ; Vol. 8, No. 7.
@article{090d8ca0c51b4668b76297bee32f7efc,
title = "Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells",
abstract = "The migratory capability of cancer cells is one of the most important hallmarks reflecting metastatic potential. Ouabain, an endogenous cardiac glycoside produced by the adrenal gland, has been previously reported to have anti-tumor activities; however, its role in the regulation of cancer cell migration remains unknown. The present study has revealed that treatment with ouabain at physiological concentrations is able to inhibit the migratory activities of human lung cancer H292 cells. The negative effects of ouabain were found to be mediated through the suppression of migration regulatory proteins, such as focal adhesion kinase (FAK), ATP-dependent tyrosine kinase (Akt), and cell division cycle 42 (Cdc42). We found that the observed actions of ouabain were mediated via a reactive oxygen species (ROS)-dependent mechanism because the addition of ROS scavengers (N-acetylcysteine and glutathione) could reverse the effect of ouabain on cell migration. Furthermore, ouabain was shown to inhibit the spheroidal tumor growth and decrease the cancer cell adhesion to endothelial cells. However, the compound had no significant effect on anoikis of the cells. Together, these findings shed light on the understanding of cancer cell biology by exploring the novel function of this endogenous human substance.",
author = "Varisa Pongrakhananon and Preedakorn Chunhacha and Pithi Chanvorachote",
year = "2013",
month = "7",
day = "10",
doi = "10.1371/journal.pone.0068623",
language = "English (US)",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

TY - JOUR

T1 - Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells

AU - Pongrakhananon, Varisa

AU - Chunhacha, Preedakorn

AU - Chanvorachote, Pithi

PY - 2013/7/10

Y1 - 2013/7/10

N2 - The migratory capability of cancer cells is one of the most important hallmarks reflecting metastatic potential. Ouabain, an endogenous cardiac glycoside produced by the adrenal gland, has been previously reported to have anti-tumor activities; however, its role in the regulation of cancer cell migration remains unknown. The present study has revealed that treatment with ouabain at physiological concentrations is able to inhibit the migratory activities of human lung cancer H292 cells. The negative effects of ouabain were found to be mediated through the suppression of migration regulatory proteins, such as focal adhesion kinase (FAK), ATP-dependent tyrosine kinase (Akt), and cell division cycle 42 (Cdc42). We found that the observed actions of ouabain were mediated via a reactive oxygen species (ROS)-dependent mechanism because the addition of ROS scavengers (N-acetylcysteine and glutathione) could reverse the effect of ouabain on cell migration. Furthermore, ouabain was shown to inhibit the spheroidal tumor growth and decrease the cancer cell adhesion to endothelial cells. However, the compound had no significant effect on anoikis of the cells. Together, these findings shed light on the understanding of cancer cell biology by exploring the novel function of this endogenous human substance.

AB - The migratory capability of cancer cells is one of the most important hallmarks reflecting metastatic potential. Ouabain, an endogenous cardiac glycoside produced by the adrenal gland, has been previously reported to have anti-tumor activities; however, its role in the regulation of cancer cell migration remains unknown. The present study has revealed that treatment with ouabain at physiological concentrations is able to inhibit the migratory activities of human lung cancer H292 cells. The negative effects of ouabain were found to be mediated through the suppression of migration regulatory proteins, such as focal adhesion kinase (FAK), ATP-dependent tyrosine kinase (Akt), and cell division cycle 42 (Cdc42). We found that the observed actions of ouabain were mediated via a reactive oxygen species (ROS)-dependent mechanism because the addition of ROS scavengers (N-acetylcysteine and glutathione) could reverse the effect of ouabain on cell migration. Furthermore, ouabain was shown to inhibit the spheroidal tumor growth and decrease the cancer cell adhesion to endothelial cells. However, the compound had no significant effect on anoikis of the cells. Together, these findings shed light on the understanding of cancer cell biology by exploring the novel function of this endogenous human substance.

UR - http://www.scopus.com/inward/record.url?scp=84880034016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880034016&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0068623

DO - 10.1371/journal.pone.0068623

M3 - Article

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 7

M1 - e68623

ER -