Outbreak of Nipah-virus infection among abattoir workers in Singapore

Nicholas I. Paton, Yee Sin Leo, Sherif R. Zaki, Alexander P. Auchus, Kim En Lee, Ai Ee Ling, Suok Kai Chew, Brenda Ang, Pierre E. Rollin, T. Umapathi, Ivy Sng, Cheng Chuan Lee, Erle Lim, Thomas Ksiazek

Research output: Contribution to journalArticle

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Abstract

Background. In March 1999, an outbreak of encephalitis and pneumonia occurred in workers at an abattoir in Singapore. We describe the clinical presentation and the results of investigations in these patients. Methods. Clinical and laboratory data were collected by systemic review of the case records. Serum and cerebrospinal fluid (CSF) samples were tested for IgM antibodies to Nipah virus with an IgM capture ELISA. Reverse-transcriptase PCR was done on the CSF and tissue samples from one patient who died. Findings. Eleven patients were confirmed to have acute Nipah-virus infection based on raised IgM in serum. Nipah virus was identified by reverse-transcriptase PCR in the CSF and tissue of the patient who died. The patients were all men, with a median age of 44 years. The commonest presenting symptoms were fever, headache, and drowsiness. Eight patients presented with signs of encephalitis (decreased level of consciousness or focal neurological signs). Three patients presented with atypical pneumonia, but one later developed hallucinations and had evidence of encephalitis on CSF examination. Abnormal laboratory findings included a low lymphocyte count (nine patients), low platelet count, low serum sodium, and high aspartate aminostransferase concentration (each observed in five patients). The CSF protein was high in eight patients and white-blood-cell count was high in seven. Chest radiography showed mild interstitial shadowing in eight patients. Magnetic resonance imaging (MRI) showed focal areas of increased signal intensity, in the cortical white marker in all eight patients who were scanned. The nine patients with encephalitis received empirical treatment with intravenous aciclovir and eight survived, Interpretation. Infection with Nipah virus caused an encephalitis illness with characteristic focal areas of increased intensity seen on MRI. Lung involvement was also common, and the disease may present as an atypical pneumonia.

Original languageEnglish (US)
Pages (from-to)1253-1256
Number of pages4
JournalLancet
Volume354
Issue number9186
DOIs
StatePublished - Oct 9 1999
Externally publishedYes

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Nipah Virus
Abattoirs
Singapore
Virus Diseases
Disease Outbreaks
Encephalitis
Cerebrospinal Fluid
Immunoglobulin M
Pneumonia
Reverse Transcriptase Polymerase Chain Reaction
Serum
Magnetic Resonance Imaging
Cerebrospinal Fluid Proteins
Acyclovir
Sleep Stages
Hallucinations
Lymphocyte Count

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Paton, N. I., Leo, Y. S., Zaki, S. R., Auchus, A. P., Lee, K. E., Ling, A. E., ... Ksiazek, T. (1999). Outbreak of Nipah-virus infection among abattoir workers in Singapore. Lancet, 354(9186), 1253-1256. https://doi.org/10.1016/S0140-6736(99)04379-2

Outbreak of Nipah-virus infection among abattoir workers in Singapore. / Paton, Nicholas I.; Leo, Yee Sin; Zaki, Sherif R.; Auchus, Alexander P.; Lee, Kim En; Ling, Ai Ee; Chew, Suok Kai; Ang, Brenda; Rollin, Pierre E.; Umapathi, T.; Sng, Ivy; Lee, Cheng Chuan; Lim, Erle; Ksiazek, Thomas.

In: Lancet, Vol. 354, No. 9186, 09.10.1999, p. 1253-1256.

Research output: Contribution to journalArticle

Paton, NI, Leo, YS, Zaki, SR, Auchus, AP, Lee, KE, Ling, AE, Chew, SK, Ang, B, Rollin, PE, Umapathi, T, Sng, I, Lee, CC, Lim, E & Ksiazek, T 1999, 'Outbreak of Nipah-virus infection among abattoir workers in Singapore', Lancet, vol. 354, no. 9186, pp. 1253-1256. https://doi.org/10.1016/S0140-6736(99)04379-2
Paton NI, Leo YS, Zaki SR, Auchus AP, Lee KE, Ling AE et al. Outbreak of Nipah-virus infection among abattoir workers in Singapore. Lancet. 1999 Oct 9;354(9186):1253-1256. https://doi.org/10.1016/S0140-6736(99)04379-2
Paton, Nicholas I. ; Leo, Yee Sin ; Zaki, Sherif R. ; Auchus, Alexander P. ; Lee, Kim En ; Ling, Ai Ee ; Chew, Suok Kai ; Ang, Brenda ; Rollin, Pierre E. ; Umapathi, T. ; Sng, Ivy ; Lee, Cheng Chuan ; Lim, Erle ; Ksiazek, Thomas. / Outbreak of Nipah-virus infection among abattoir workers in Singapore. In: Lancet. 1999 ; Vol. 354, No. 9186. pp. 1253-1256.
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abstract = "Background. In March 1999, an outbreak of encephalitis and pneumonia occurred in workers at an abattoir in Singapore. We describe the clinical presentation and the results of investigations in these patients. Methods. Clinical and laboratory data were collected by systemic review of the case records. Serum and cerebrospinal fluid (CSF) samples were tested for IgM antibodies to Nipah virus with an IgM capture ELISA. Reverse-transcriptase PCR was done on the CSF and tissue samples from one patient who died. Findings. Eleven patients were confirmed to have acute Nipah-virus infection based on raised IgM in serum. Nipah virus was identified by reverse-transcriptase PCR in the CSF and tissue of the patient who died. The patients were all men, with a median age of 44 years. The commonest presenting symptoms were fever, headache, and drowsiness. Eight patients presented with signs of encephalitis (decreased level of consciousness or focal neurological signs). Three patients presented with atypical pneumonia, but one later developed hallucinations and had evidence of encephalitis on CSF examination. Abnormal laboratory findings included a low lymphocyte count (nine patients), low platelet count, low serum sodium, and high aspartate aminostransferase concentration (each observed in five patients). The CSF protein was high in eight patients and white-blood-cell count was high in seven. Chest radiography showed mild interstitial shadowing in eight patients. Magnetic resonance imaging (MRI) showed focal areas of increased signal intensity, in the cortical white marker in all eight patients who were scanned. The nine patients with encephalitis received empirical treatment with intravenous aciclovir and eight survived, Interpretation. Infection with Nipah virus caused an encephalitis illness with characteristic focal areas of increased intensity seen on MRI. Lung involvement was also common, and the disease may present as an atypical pneumonia.",
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