Overexpression of Evi-1 oncoprotein represses TGF-β signaling in colorectal cancer

Xiyun Deng, Yanna Cao, Yan Liu, Fazhi Li, Kamalanathan Sambandam, Srinivasan Rajaraman, Archibald S. Perkins, Alan P. Fields, Mark Hellmich, Courtney Townsend, E. Aubrey Thompson, Tien C. Ko

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Human colorectal cancer (CRC) cells are resistant to the anti-proliferative effect of transforming growth factor-β (TGF-β), suggesting that disruption of TGF-β signaling plays an important role in colorectal carcinogenesis. Ecotropic virus integration site-1 (Evi-1) oncoprotein represses TGF-β signaling by interacting with Smads, but its role in CRC has not been established. The purpose of this study is to determine whether Evi-1 plays role(s) in CRCs and to characterize Evi-1 transcript(s) in CRCs. Evi-1 was overexpressed in 53% of human CRC samples, 100% of colon adenoma samples, and 100% of human colon cancer cell lines tested. Using 5′ RACE, we cloned a novel Evi-1 transcript (Evi-1e) from a human CRC tissue and found that this novel transcript was expressed at a higher level in CRC tissues than in normal tissues and was the major Evi-1 transcript in CRCs. Transient Evi-1 transfection inhibited TGF-β-induced transcriptional activity and reversed the growth inhibitory effect of TGF-β in MC-26 mouse colon cancer cells. In conclusion, we have identified overexpression of Evi-1 oncoprotein as a novel mechanism by which a subset of human CRCs may escape TGF-β regulation. We have also identified a novel Evi-1 transcript, Evi-1e, as the major Evi-1 transcript expressed in human CRCs. © 2011 Wiley Periodicals, Inc.

Original languageEnglish (US)
Pages (from-to)255-264
Number of pages10
JournalMolecular Carcinogenesis
Volume52
Issue number4
DOIs
StatePublished - Apr 2013

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Virus Integration
Oncogene Proteins
Transforming Growth Factors
Colorectal Neoplasms
Colonic Neoplasms
Adenoma
Transfection
Colon
Carcinogenesis

Keywords

  • Colorectal cancer
  • Ecotropic virus integration site-1
  • Growth inhibition
  • Rapid amplification of cDNA ends
  • Smad proteins
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Deng, X., Cao, Y., Liu, Y., Li, F., Sambandam, K., Rajaraman, S., ... Ko, T. C. (2013). Overexpression of Evi-1 oncoprotein represses TGF-β signaling in colorectal cancer. Molecular Carcinogenesis, 52(4), 255-264. https://doi.org/10.1002/mc.21852

Overexpression of Evi-1 oncoprotein represses TGF-β signaling in colorectal cancer. / Deng, Xiyun; Cao, Yanna; Liu, Yan; Li, Fazhi; Sambandam, Kamalanathan; Rajaraman, Srinivasan; Perkins, Archibald S.; Fields, Alan P.; Hellmich, Mark; Townsend, Courtney; Thompson, E. Aubrey; Ko, Tien C.

In: Molecular Carcinogenesis, Vol. 52, No. 4, 04.2013, p. 255-264.

Research output: Contribution to journalArticle

Deng, X, Cao, Y, Liu, Y, Li, F, Sambandam, K, Rajaraman, S, Perkins, AS, Fields, AP, Hellmich, M, Townsend, C, Thompson, EA & Ko, TC 2013, 'Overexpression of Evi-1 oncoprotein represses TGF-β signaling in colorectal cancer', Molecular Carcinogenesis, vol. 52, no. 4, pp. 255-264. https://doi.org/10.1002/mc.21852
Deng, Xiyun ; Cao, Yanna ; Liu, Yan ; Li, Fazhi ; Sambandam, Kamalanathan ; Rajaraman, Srinivasan ; Perkins, Archibald S. ; Fields, Alan P. ; Hellmich, Mark ; Townsend, Courtney ; Thompson, E. Aubrey ; Ko, Tien C. / Overexpression of Evi-1 oncoprotein represses TGF-β signaling in colorectal cancer. In: Molecular Carcinogenesis. 2013 ; Vol. 52, No. 4. pp. 255-264.
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abstract = "Human colorectal cancer (CRC) cells are resistant to the anti-proliferative effect of transforming growth factor-β (TGF-β), suggesting that disruption of TGF-β signaling plays an important role in colorectal carcinogenesis. Ecotropic virus integration site-1 (Evi-1) oncoprotein represses TGF-β signaling by interacting with Smads, but its role in CRC has not been established. The purpose of this study is to determine whether Evi-1 plays role(s) in CRCs and to characterize Evi-1 transcript(s) in CRCs. Evi-1 was overexpressed in 53{\%} of human CRC samples, 100{\%} of colon adenoma samples, and 100{\%} of human colon cancer cell lines tested. Using 5′ RACE, we cloned a novel Evi-1 transcript (Evi-1e) from a human CRC tissue and found that this novel transcript was expressed at a higher level in CRC tissues than in normal tissues and was the major Evi-1 transcript in CRCs. Transient Evi-1 transfection inhibited TGF-β-induced transcriptional activity and reversed the growth inhibitory effect of TGF-β in MC-26 mouse colon cancer cells. In conclusion, we have identified overexpression of Evi-1 oncoprotein as a novel mechanism by which a subset of human CRCs may escape TGF-β regulation. We have also identified a novel Evi-1 transcript, Evi-1e, as the major Evi-1 transcript expressed in human CRCs. {\circledC} 2011 Wiley Periodicals, Inc.",
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AU - Perkins, Archibald S.

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