Overexpression of heat shock factor 1 inhibits butyrate-induced differentiation in colon cancer cells

Jiyang Cai, Ward G. Kirlin, Yan Chen, Xuexian Yan, Dean P. Jones, Alan C. Sartorelli

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Produced by dietary fiber, butyrate is a potential chemopreventive agent against colon cancer. It stimulates proliferation of normal colonic epithelial cells but induces growth inhibition, differentiation, apoptosis, or a combination of effects in colon carcinoma cells. In this study, we used cDNA membrane arrays and real-time reverse transcriptase-polymerase chain reaction to identify stress genes that were differentially regulated by sodium butyrate (NaB) in HT 29 human colon carcinoma cells. The results indicated that a group of heat shock protein (hsp) genes were upregulated by 3 mM NaB within the first 24 hours of exposure. Because the transcription of hsp genes is under the control of heat shock factors (HSFs), we measured the effects of overexpressed HSF-1 on the responses of HT 29 cells to NaB. Overexpression of HSF-1 inhibited NaB-induced differentiation as measured by alkaline phosphatase activity and carcinoembryonic antigen expression. These results suggest that increased expression of HSFs and Hsps might render colon carcinoma cells resistant to the chemopreventive effects of butyrate.

Original languageEnglish (US)
Pages (from-to)199-207
Number of pages9
JournalCell Stress and Chaperones
Volume11
Issue number3
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Butyrates
Colonic Neoplasms
Shock
Hot Temperature
Cells
Colon
Genes
Heat-Shock Proteins
Carcinoma
HT29 Cells
Butyric Acid
Polymerase chain reaction
RNA-Directed DNA Polymerase
Carcinoembryonic Antigen
Dietary Fiber
Transcription
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Alkaline Phosphatase
Real-Time Polymerase Chain Reaction

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Overexpression of heat shock factor 1 inhibits butyrate-induced differentiation in colon cancer cells. / Cai, Jiyang; Kirlin, Ward G.; Chen, Yan; Yan, Xuexian; Jones, Dean P.; Sartorelli, Alan C.

In: Cell Stress and Chaperones, Vol. 11, No. 3, 2006, p. 199-207.

Research output: Contribution to journalArticle

Cai, Jiyang ; Kirlin, Ward G. ; Chen, Yan ; Yan, Xuexian ; Jones, Dean P. ; Sartorelli, Alan C. / Overexpression of heat shock factor 1 inhibits butyrate-induced differentiation in colon cancer cells. In: Cell Stress and Chaperones. 2006 ; Vol. 11, No. 3. pp. 199-207.
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