Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6

Y. Ye, M. Falzon, P. K. Seitz, C. W. Cooper

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1-34), (67-86), or (107-139) to growth medium did not affect proliferation of cells grown in either high (10% Nu-Serum) or low serum (1% Nu-Serum). However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and 3H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate 3H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects.

Original languageEnglish (US)
Pages (from-to)169-174
Number of pages6
JournalRegulatory Peptides
Volume99
Issue number2-3
DOIs
StatePublished - Jun 15 2001

Keywords

  • GI epithelium
  • Nuclear localization signal
  • Transfection

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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