Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6

Y. Ye; M. Falzon; P. K. Seitz; C. W. Cooper

doi:10.1016/S0167-0115(01)00248-8

Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6

Y. Ye, M. Falzon, P. K. Seitz, C. W. Cooper

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1-34), (67-86), or (107-139) to growth medium did not affect proliferation of cells grown in either high (10% Nu-Serum) or low serum (1% Nu-Serum). However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and ³H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate ³H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects.

Original language	English (US)
Pages (from-to)	169-174
Number of pages	6
Journal	Regulatory Peptides
Volume	99
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-0115(01)00248-8
State	Published - Jun 15 2001
Externally published	Yes

Keywords

GI epithelium
Nuclear localization signal
Transfection

ASJC Scopus subject areas

Physiology
Biochemistry
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/S0167-0115(01)00248-8

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title = "Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6",

abstract = "The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1-34), (67-86), or (107-139) to growth medium did not affect proliferation of cells grown in either high (10% Nu-Serum) or low serum (1% Nu-Serum). However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and 3H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate 3H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects.",

keywords = "GI epithelium, Nuclear localization signal, Transfection",

author = "Y. Ye and M. Falzon and Seitz, {P. K.} and Cooper, {C. W.}",

note = "Funding Information: This work was supported by NIH grant DK35608.",

year = "2001",

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doi = "10.1016/S0167-0115(01)00248-8",

language = "English (US)",

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pages = "169--174",

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T1 - Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6

AU - Ye, Y.

AU - Falzon, M.

AU - Seitz, P. K.

AU - Cooper, C. W.

N1 - Funding Information: This work was supported by NIH grant DK35608.

PY - 2001/6/15

Y1 - 2001/6/15

N2 - The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1-34), (67-86), or (107-139) to growth medium did not affect proliferation of cells grown in either high (10% Nu-Serum) or low serum (1% Nu-Serum). However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and 3H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate 3H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects.

AB - The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1-34), (67-86), or (107-139) to growth medium did not affect proliferation of cells grown in either high (10% Nu-Serum) or low serum (1% Nu-Serum). However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and 3H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate 3H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects.

KW - GI epithelium

KW - Nuclear localization signal

KW - Transfection

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Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6

Abstract

Keywords

ASJC Scopus subject areas

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