Oxanine DNA glycosylase activities in mammalian systems

Liang Dong, Lisiane B. Meira, Tapas Hazra, Leona D. Samson, Weiguo Cao

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

DNA bases carrying an exocyclic amino group, namely adenine (A), guanine (G) and cytosine (C), encounter deamination under nitrosative stress. Oxanine (O), derived from deamination of guanine, is a cytotoxic and potentially mutagenic lesion and studies of its enzymatic repair are limited. Previously, we reported that the murine alkyladenine glycosylase (Aag) acts as an oxanine DNA glycosylase (JBC (2004), 279: 38177). Here, we report our recent findings on additional oxanine DNA glycosylase (ODG) activities in Aag knockout mouse tissues and other mammalian tissues. Analysis of the partially purified proteins from the mammalian cell extracts indicated the existence of ODG enzymes in addition to Aag. Data obtained from oxanine DNA cleavage assays using purified human glycosylases demonstrated that two known glycosylases, hNEIL1 and hSMUG1, contained weak but detectable ODG activities. ODG activity was the highest in hAAG and lowest in hSMUG1.

Original languageEnglish (US)
Pages (from-to)128-134
Number of pages7
JournalDNA Repair
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2008

Fingerprint

DNA Glycosylases
Deamination
Guanine
Tissue
DNA Cleavage
Cytosine
DNA
Adenine
oxanine
Cell Extracts
Knockout Mice
Assays
Repair
Cells
Enzymes

Keywords

  • AAG
  • Deamination
  • Mammals
  • NEIL1
  • Oxanine DNA glycosylase
  • SMUG1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Oxanine DNA glycosylase activities in mammalian systems. / Dong, Liang; Meira, Lisiane B.; Hazra, Tapas; Samson, Leona D.; Cao, Weiguo.

In: DNA Repair, Vol. 7, No. 1, 01.01.2008, p. 128-134.

Research output: Contribution to journalArticle

Dong, L, Meira, LB, Hazra, T, Samson, LD & Cao, W 2008, 'Oxanine DNA glycosylase activities in mammalian systems', DNA Repair, vol. 7, no. 1, pp. 128-134. https://doi.org/10.1016/j.dnarep.2007.09.004
Dong, Liang ; Meira, Lisiane B. ; Hazra, Tapas ; Samson, Leona D. ; Cao, Weiguo. / Oxanine DNA glycosylase activities in mammalian systems. In: DNA Repair. 2008 ; Vol. 7, No. 1. pp. 128-134.
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