Oxidant tone regulates RANTES gene expression in airway epithelial cells infected with respiratory syncytial virus. Role in viral-induced interferon regulatory factor activation

Antonella Casola, Nathalie Burger, Tianshuang Liu, Mohammad Jamaluddin, Allan R. Brasier, Roberto Garofalo

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Respiratory syncytial virus (RSV) produces intense pulmonary inflammation, in part, through its ability to induce chemokine synthesis in infected airway epithelial cells. RANTES (regulated upon activation, normal T-cells expressed and secreted) is a CC chemokine which recruits and activates monocytes, lymphocytes, and eosinophils, all cell types present in the lung inflammatory infiltrate induced by RSV infection. In this study we investigated the role of reactive oxygen species in the induction of RANTES gene expression in human type II alveolar epithelial cells (A549), following RSV infection. Our results indicate that RSV infection of airway epithelial cells rapidly induces reactive oxygen species production, prior to RANTES expression, as measured by oxidation of 2′,7′-dichlorofluorescein. Pretreatment of airway epithelial cells with the antioxidant butylated hydroxyanisol (BHA), as well a panel of chemically unrelated antioxidants, blocks RSV-induced RANTES gene expression and protein secretion. This effect is mediated through the ability of BHA to inhibit RSV-induced interferon regulatory factor binding to the RANTES promoter interferon-stimulated responsive element, that is absolutely required for inducible RANTES promoter activation. BHA inhibits de novo interferon regulator factor (IRF)-1 and -7 gene expression and protein synthesis, and IRF-3 nuclear translocation. Together, these data indicates that a redox-sensitive pathway is involved in RSV-induced IRF activation, an event necessary for RANTES gene expression.

Original languageEnglish (US)
Pages (from-to)19715-19722
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number23
DOIs
StatePublished - Jun 8 2001

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Interferon Regulatory Factors
Respiratory Syncytial Viruses
T-cells
Viruses
Oxidants
Gene expression
Epithelial Cells
Chemical activation
T-Lymphocytes
Gene Expression
Respiratory Syncytial Virus Infections
Interferons
Reactive Oxygen Species
Antioxidants
Alveolar Epithelial Cells
CC Chemokines
viral interferon regulatory factors
Chemokines
Eosinophils
Lymphocytes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Oxidant tone regulates RANTES gene expression in airway epithelial cells infected with respiratory syncytial virus. Role in viral-induced interferon regulatory factor activation. / Casola, Antonella; Burger, Nathalie; Liu, Tianshuang; Jamaluddin, Mohammad; Brasier, Allan R.; Garofalo, Roberto.

In: Journal of Biological Chemistry, Vol. 276, No. 23, 08.06.2001, p. 19715-19722.

Research output: Contribution to journalArticle

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