Oxidation of mitochondrial and nuclear proteins by endogenously produced peroxynitrite in immunostimulated macrophages

M. O'Connor, A. L. Salzman, Csaba Szabo

Research output: Contribution to journalArticle

Abstract

Endogenously produced peroxynitrite ia toxic oxidant produced from NO and superoxide) suppresses mitochondrial respiration and induces DNA strand breakage in immunostimulated macrophages. In the present study we investigated the role of endogenous and exogenous nitric oxide (NO) and peroxynitrite in the process of protein oxidation, as measured by the detection of 2,4 dinitrophenylhydrazine-reactive carbonyls. In J774 macrophages. Exogenously added hydrogen peroxide and peroxynitrite, but not NO, caused a marked increase in protein oxidation in the macrophages. Moreover, immunostimulation of the macrophages with bacterial lipopolysaccharide and gamma-interferon (LPS/IFN) resulted in a marked increase in the protein oxidation of these cells. Fractionation of nuclei and mitochondrial from these cells, followed by detection of 2.4 dinitrophenylhydrazine-reactive carbonyls revealed that immunostimulation causes protein oxidation of a large number of mitochondrial as well as nuclear proteins. The inhibitor of NO synthase, NG-methyl-L-arginine (L-NMA, 3 mM) and the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP, (300 μM) both inhibited the increase in protein oxidation in response to LPS/IFN. These data demonstrate that immunostimulation induced marked protein oxidation in cultured macrophages. The protective effect of L-NMA as well as MnTBAP suggest that endogenous peroxynitrite (formed from the reaction of superoxide with NO, the latter produced by inducible NO synthase) induces protein oxidation in the immunostimulated cells.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

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Peroxynitrous Acid
Macrophages
Mitochondrial Proteins
nuclear proteins
Nuclear Proteins
macrophages
oxidation
immunostimulation (physiological)
Oxidation
nitric oxide
Nitric Oxide
Proteins
proteins
Immunization
interferon-gamma
Superoxides
superoxide anion
lipopolysaccharides
Interferon-gamma
Lipopolysaccharides

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Oxidation of mitochondrial and nuclear proteins by endogenously produced peroxynitrite in immunostimulated macrophages. / O'Connor, M.; Salzman, A. L.; Szabo, Csaba.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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