Oxidative and nitrosative stress in trichloroethene-mediated autoimmune response

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Reactive oxygen and nitrogen species (RONS) are implicated in the pathogenesis of several autoimmune diseases. Also, increased lipid peroxidation and protein nitration are reported in systemic autoimmune diseases. Lipid peroxidation-derived aldehydes (LPDAs) such as malondialdehyde (MDA) and 4-hydroxynonenal (HNE) are highly reactive and bind proteins covalently, but their potential to elicit an autoimmune response and contribution to disease pathogenesis remain unclear. Similarly, nitration of protein could also contribute to disease pathogenesis. To assess the status of lipid peroxidation and/or RONS, autoimmune-prone female MRL+/+ mice (5-week old) were treated with trichloroethene (TCE), an environmental contaminant known to induce autoimmune response, for 48 weeks (0.5 mg/ml via drinking water), and formation of antibodies to LPDA-protein adducts was followed in the sera of control and TCE-treated mice. TCE treatment led to greater formation of both anti-MDA- and -HNE-protein adduct antibodies and higher serum iNOS and nitrotyrosine levels. The increase in TCE-induced oxidative stress was associated with increases in anti-nuclear-, anti-ssDNA- and anti-dsDNA-antibodies. These findings suggest that TCE exposure not only leads to oxidative/nitrosative stress, but is also associated with induction/exacerbation of autoimmune response in MRL+/+ mice. Further interventional studies are needed to establish a causal role of RONS in TCE-mediated autoimmunity.

Original languageEnglish (US)
Pages (from-to)186-193
Number of pages8
JournalToxicology
Volume229
Issue number3
DOIs
StatePublished - Jan 18 2007

Fingerprint

Trichloroethylene
Autoimmunity
Oxidative Stress
Reactive Nitrogen Species
Lipid Peroxidation
Nitration
Lipids
Reactive Oxygen Species
Oxidative stress
Proteins
Malondialdehyde
Aldehydes
Autoimmune Diseases
Antibodies
Serum
Drinking Water
Antibody Formation
Anti-Idiotypic Antibodies
Impurities

Keywords

  • Autoimmune diseases
  • HNE
  • iNOS
  • MDA
  • Nitrotyrosine
  • Oxidative stress
  • Trichloroethene

ASJC Scopus subject areas

  • Toxicology

Cite this

Oxidative and nitrosative stress in trichloroethene-mediated autoimmune response. / Wang, Gangduo; Cai, Ping; Ansari, Ghulam; Khan, M.

In: Toxicology, Vol. 229, No. 3, 18.01.2007, p. 186-193.

Research output: Contribution to journalArticle

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