Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats

P. Khanna, L. Wang, R. J. Perez-Polo, Naseem Ansari

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This study examines the mRNA expression and enzyme activity of oxidative defense enzymes during the course of streptozotocin-induced hyperglycemic cataract development Diabetes was produced in 5-wk-old male Sprague-Dawley rats by administering streptozotocin ip and mRNA expression and enzyme activity were monitored on d 4, 8, 12, 16, 20, 40, 60, and 80; concomitantly, the onset and progress of cataract was followed by digital image analysis. Peak enzyme activity and mRNA expression were attained between d 20 and 40. Although catalase and glutathione peroxidase maintained high levels of mRNA expression through d 60, induction of CuZu-superoxide dismutase was transient with the activity and mRNA levels returning to baseline values by d 40. There was a pronounced increase in aldose reductase activity, which gradually declined to basal levels by d 60; however, the mRNA levels remained unaltered. Other changes included a progressive loss of lenticular transparency, which declined to 40% of control by d 80. The role of antioxidant defense enzymes and, more interestingly, aldose reductase in combating oxidative stress in diabetic cataractogenesis is discussed.

Original languageEnglish (US)
Pages (from-to)541-555
Number of pages15
JournalJournal of Toxicology and Environmental Health
Volume51
Issue number6
DOIs
StatePublished - 1997

Fingerprint

Enzyme activity
Lenses
enzyme activity
Rats
Messenger RNA
Enzymes
enzyme
Aldehyde Reductase
diabetes
Streptozocin
digital image
transparency
image analysis
Cataract
antioxidant
Oxidative stress
Medical problems
Glutathione Peroxidase
Antioxidants
Catalase

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats. / Khanna, P.; Wang, L.; Perez-Polo, R. J.; Ansari, Naseem.

In: Journal of Toxicology and Environmental Health, Vol. 51, No. 6, 1997, p. 541-555.

Research output: Contribution to journalArticle

@article{5bbb0a84558e46e2ab093edbab18a135,
title = "Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats",
abstract = "This study examines the mRNA expression and enzyme activity of oxidative defense enzymes during the course of streptozotocin-induced hyperglycemic cataract development Diabetes was produced in 5-wk-old male Sprague-Dawley rats by administering streptozotocin ip and mRNA expression and enzyme activity were monitored on d 4, 8, 12, 16, 20, 40, 60, and 80; concomitantly, the onset and progress of cataract was followed by digital image analysis. Peak enzyme activity and mRNA expression were attained between d 20 and 40. Although catalase and glutathione peroxidase maintained high levels of mRNA expression through d 60, induction of CuZu-superoxide dismutase was transient with the activity and mRNA levels returning to baseline values by d 40. There was a pronounced increase in aldose reductase activity, which gradually declined to basal levels by d 60; however, the mRNA levels remained unaltered. Other changes included a progressive loss of lenticular transparency, which declined to 40{\%} of control by d 80. The role of antioxidant defense enzymes and, more interestingly, aldose reductase in combating oxidative stress in diabetic cataractogenesis is discussed.",
author = "P. Khanna and L. Wang and Perez-Polo, {R. J.} and Naseem Ansari",
year = "1997",
doi = "10.1080/009841097159827",
language = "English (US)",
volume = "51",
pages = "541--555",
journal = "Journal of Toxicology and Environmental Health - Part A: Current Issues",
issn = "1528-7394",
publisher = "Taylor and Francis Ltd.",
number = "6",

}

TY - JOUR

T1 - Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats

AU - Khanna, P.

AU - Wang, L.

AU - Perez-Polo, R. J.

AU - Ansari, Naseem

PY - 1997

Y1 - 1997

N2 - This study examines the mRNA expression and enzyme activity of oxidative defense enzymes during the course of streptozotocin-induced hyperglycemic cataract development Diabetes was produced in 5-wk-old male Sprague-Dawley rats by administering streptozotocin ip and mRNA expression and enzyme activity were monitored on d 4, 8, 12, 16, 20, 40, 60, and 80; concomitantly, the onset and progress of cataract was followed by digital image analysis. Peak enzyme activity and mRNA expression were attained between d 20 and 40. Although catalase and glutathione peroxidase maintained high levels of mRNA expression through d 60, induction of CuZu-superoxide dismutase was transient with the activity and mRNA levels returning to baseline values by d 40. There was a pronounced increase in aldose reductase activity, which gradually declined to basal levels by d 60; however, the mRNA levels remained unaltered. Other changes included a progressive loss of lenticular transparency, which declined to 40% of control by d 80. The role of antioxidant defense enzymes and, more interestingly, aldose reductase in combating oxidative stress in diabetic cataractogenesis is discussed.

AB - This study examines the mRNA expression and enzyme activity of oxidative defense enzymes during the course of streptozotocin-induced hyperglycemic cataract development Diabetes was produced in 5-wk-old male Sprague-Dawley rats by administering streptozotocin ip and mRNA expression and enzyme activity were monitored on d 4, 8, 12, 16, 20, 40, 60, and 80; concomitantly, the onset and progress of cataract was followed by digital image analysis. Peak enzyme activity and mRNA expression were attained between d 20 and 40. Although catalase and glutathione peroxidase maintained high levels of mRNA expression through d 60, induction of CuZu-superoxide dismutase was transient with the activity and mRNA levels returning to baseline values by d 40. There was a pronounced increase in aldose reductase activity, which gradually declined to basal levels by d 60; however, the mRNA levels remained unaltered. Other changes included a progressive loss of lenticular transparency, which declined to 40% of control by d 80. The role of antioxidant defense enzymes and, more interestingly, aldose reductase in combating oxidative stress in diabetic cataractogenesis is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0030804998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030804998&partnerID=8YFLogxK

U2 - 10.1080/009841097159827

DO - 10.1080/009841097159827

M3 - Article

C2 - 9242227

AN - SCOPUS:0030804998

VL - 51

SP - 541

EP - 555

JO - Journal of Toxicology and Environmental Health - Part A: Current Issues

JF - Journal of Toxicology and Environmental Health - Part A: Current Issues

SN - 1528-7394

IS - 6

ER -