Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats

P. Khanna, L. Wang, R. J. Perez-Polo, Naseem Ansari

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16 Scopus citations

Abstract

This study examines the mRNA expression and enzyme activity of oxidative defense enzymes during the course of streptozotocin-induced hyperglycemic cataract development Diabetes was produced in 5-wk-old male Sprague-Dawley rats by administering streptozotocin ip and mRNA expression and enzyme activity were monitored on d 4, 8, 12, 16, 20, 40, 60, and 80; concomitantly, the onset and progress of cataract was followed by digital image analysis. Peak enzyme activity and mRNA expression were attained between d 20 and 40. Although catalase and glutathione peroxidase maintained high levels of mRNA expression through d 60, induction of CuZu-superoxide dismutase was transient with the activity and mRNA levels returning to baseline values by d 40. There was a pronounced increase in aldose reductase activity, which gradually declined to basal levels by d 60; however, the mRNA levels remained unaltered. Other changes included a progressive loss of lenticular transparency, which declined to 40% of control by d 80. The role of antioxidant defense enzymes and, more interestingly, aldose reductase in combating oxidative stress in diabetic cataractogenesis is discussed.

Original languageEnglish (US)
Pages (from-to)541-555
Number of pages15
JournalJournal of Toxicology and Environmental Health
Volume51
Issue number6
DOIs
StatePublished - 1997

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ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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