Oxidative defense enzyme activity and mrna levels in lenses of diabetic rats

Peeyush Khanna, Lifei Wang, Regino J. Perez-Polo, Naseem H. Ansari

    Research output: Contribution to journalArticle

    16 Scopus citations

    Abstract

    This study examines the mRNA expression and enzyme activity of oxidative defense enzymes during the course of streptozotocin-induced hyperglycemic cataract development. Diabetes was produced in 5-wk-old male Sprague-Dawley rats by administering streptozotocin ip and mRNA expression and enzyme activity were monitored on d 4, 8, 12, 16, 20, 40, 60, and 80; concomitantly, the onset and progress of cataract was followed by digital image analysis. Peak enzyme activity and mRNA expression were attained between d 20 and 40. Although catalase and glutathione peroxidase maintained high levels of mRNA expression through d 60, induction of CuZu-superoxide dismutase was transient, with the activity and mRNA levels returning to baseline values by d 40. There was a pronounced increase in aldose reductase activity, which gradually declined to basal levels byd 60; however, the mRNA levels remained unaltered. Other changes included a progressive loss of lenticular transparency, which declined to 40% of control by d 80. The role of antioxidant defense enzymes and, more interestingly, aldose reductase in combating oxidative stress in diabetic cataractogenesis is discussed.

    Original languageEnglish (US)
    Pages (from-to)541-555
    Number of pages15
    JournalJournal of Toxicology and Environmental Health
    Volume51
    Issue number6
    DOIs
    StatePublished - Aug 1997

    ASJC Scopus subject areas

    • Toxicology
    • Pollution

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