Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts

Courtney E. Cross, Mai F. Tolba, Catherine M. Rondelli, Meixiang Xu, Sherif Abdel-Rahman

Research output: Contribution to journalArticle

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Abstract

The relationship between oxidative stress and miRNA changes in placenta as a potential mechanism involved in preeclampsia (PE) is not fully elucidated. We investigated the impact of oxidative stress on miRNAs and mRNA expression profiles of genes associated with PE in villous 3A first trimester trophoblast cells exposed to H<inf>2</inf>O<inf>2</inf> at 12 different concentrations (0-1 mM) for 0.5, 4, 24, and 48 h. Cytotoxicity, determined using the SRB assay, was used to calculate the IC<inf>50</inf> of H<inf>2</inf>O<inf>2</inf>. RNA was extracted after 4 h exposure to H<inf>2</inf>O<inf>2</inf> for miRNA and gene expression profiling. H<inf>2</inf>O<inf>2</inf> exerted a concentration-and time-dependent cytotoxicity on 3A trophoblast cells. Short-term exposure of 3A cells to low concentration of H<inf>2</inf>O<inf>2</inf> (5% of IC<inf>50</inf>) significantly altered miRNA profile as evidenced by significant changes in 195 out of 595 evaluable miRNAs. Tool for annotations of microRNAs (TAM) analysis indicated that these altered miRNAs fall into 43 clusters and 34 families, with 41 functions identified. Exposure to H<inf>2</inf>O<inf>2</inf> altered mRNA expression of 22 out of 84 key genes involved in dysregulation of placental development. In conclusion, short-term exposure of villous first trimester trophoblasts to low concentrations of H<inf>2</inf>O<inf>2</inf> significantly alters miRNA profile and expression of genes implicated in placental development.

Original languageEnglish (US)
Article number257090
JournalBioMed Research International
Volume2015
DOIs
StatePublished - 2015

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Oxidative stress
Trophoblasts
First Pregnancy Trimester
MicroRNAs
Transcriptome
Gene expression
Oxidative Stress
Placentation
Genes
Cytotoxicity
Pre-Eclampsia
Inhibitory Concentration 50
Messenger RNA
Gene Expression Profiling
Placenta
Assays
RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts. / Cross, Courtney E.; Tolba, Mai F.; Rondelli, Catherine M.; Xu, Meixiang; Abdel-Rahman, Sherif.

In: BioMed Research International, Vol. 2015, 257090, 2015.

Research output: Contribution to journalArticle

Cross, Courtney E. ; Tolba, Mai F. ; Rondelli, Catherine M. ; Xu, Meixiang ; Abdel-Rahman, Sherif. / Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts. In: BioMed Research International. 2015 ; Vol. 2015.
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