Oxidative stress alters specific membrane currents in isolated cardiac myocytes

A. Bhatnagar, Satish Srivastava, G. Szabo

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

To evaluate the effects of oxidative stress on cardiac membrane currents, single cells from frog ventricle were exposed to tert-butyl hydroperoxide (t-BHP). Incubation of these cells with 2 mM t-BHP causes a rapid depletion of cellular glutathione, followed by a more gradual increase in the contents of malonaldialdehyde and conjugated dienes. Effects of this rapidly evolving oxidative stress were studied on sodium, calcium, and potassium currents of isolated ventricular cells. t-BHP caused a progressive decrease in the magnitude of sodium current obtained on depolarization from a holding potential of -85 mV, which was accompanied by a shift in the reversal potential toward more negative potentials. The voltage dependence of the steady-state parameters for activation and inactivation was shifted, such that in peroxide-exposed cells, there was a greater overlap of activation and inactivation parameters, which would be expected to result in an increased window current. In addition, in the presence of t-BHP, the time constant for activation was decreased at most depolarizing potentials, whereas the time constant for inactivation was increased. The resultant sodium current transients were, therefore, slower in the presence of the peroxide because of slower inactivation. Prolonged exposure of the cells to t-BHP led to a complete and selective inhibition of the Na+ current. However, even when all the Na+ current was inhibited, the K+ and Ca2+ currents remained essentially unaltered. Also, no large outward currents were observed at this stage, indicating that ATP concentration was not drastically decreased. The barrier properties of plasma membrane remained intact, as it was possible to form gigohm seals between the patch pipette and the plasma membrane of cells treated with 2-14 mM t-BHP for up to 30 minutes. These results account for the proarrhythmic effects of free radicals and oxidative stress on cardiac tissues.

Original languageEnglish (US)
Pages (from-to)535-549
Number of pages15
JournalCirculation Research
Volume67
Issue number3
StatePublished - 1990
Externally publishedYes

Fingerprint

tert-Butylhydroperoxide
Cardiac Myocytes
Oxidative Stress
Membranes
Sodium
Peroxides
Cell Membrane
Plasma Cells
Anura
Free Radicals
Glutathione
Potassium
Adenosine Triphosphate
Calcium

Keywords

  • free radicals
  • ion channels
  • oxidative stress
  • reperfusion
  • ventricular myocytes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Bhatnagar, A., Srivastava, S., & Szabo, G. (1990). Oxidative stress alters specific membrane currents in isolated cardiac myocytes. Circulation Research, 67(3), 535-549.

Oxidative stress alters specific membrane currents in isolated cardiac myocytes. / Bhatnagar, A.; Srivastava, Satish; Szabo, G.

In: Circulation Research, Vol. 67, No. 3, 1990, p. 535-549.

Research output: Contribution to journalArticle

Bhatnagar, A, Srivastava, S & Szabo, G 1990, 'Oxidative stress alters specific membrane currents in isolated cardiac myocytes', Circulation Research, vol. 67, no. 3, pp. 535-549.
Bhatnagar, A. ; Srivastava, Satish ; Szabo, G. / Oxidative stress alters specific membrane currents in isolated cardiac myocytes. In: Circulation Research. 1990 ; Vol. 67, No. 3. pp. 535-549.
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