Oxidative stress and antioxidant response in a thermotolerant yeast

Jorge A. Mejía-Barajas, Rocío Montoya-Pérez, Rafael Salgado-Garciglia, Leopoldo Aguilera-Aguirre, Christian Cortés-Rojo, Ricardo Mejía-Zepeda, Melchor Arellano-Plaza, Alfredo Saavedra-Molina

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Stress tolerance is a key attribute that must be considered when using yeast cells for industrial applications. High temperature is one factor that can cause stress in yeast. High environmental temperature in particular may exert a natural selection pressure to evolve yeasts into thermotolerant strains. In the present study, three yeasts (Saccharomyces cerevisiae, MC4, and Kluyveromyces marxianus, OFF1 and SLP1) isolated from hot environments were exposed to increased temperatures and were then compared with a laboratory yeast strain. Their resistance to high temperature, oxidative stress, and antioxidant response were evaluated, along with the fatty acid composition of their cell membranes. The SLP1 strain showed a higher specific growth rate, biomass yield, and biomass volumetric productivity while also showing lower duplication time, reactive oxygen species (ROS) production, and lipid peroxidation. In addition, the SLP1 strain demonstrated more catalase activity after temperature was increased, and this strain also showed membranes enriched in saturated fatty acids. It is concluded that the SLP1 yeast strain is a thermotolerant yeast with less oxidative stress and a greater antioxidant response. Therefore, this strain could be used for fermentation at high temperatures.

Original languageEnglish (US)
Pages (from-to)326-332
Number of pages7
JournalBrazilian Journal of Microbiology
Volume48
Issue number2
DOIs
StatePublished - Apr 1 2017

Fingerprint

Oxidative Stress
Antioxidants
Yeasts
Temperature
Biomass
Fatty Acids
Kluyveromyces
Genetic Selection
Catalase
Lipid Peroxidation
Fermentation
Saccharomyces cerevisiae
Reactive Oxygen Species
Cell Membrane
Pressure
Membranes
Growth

Keywords

  • Increased temperature
  • Oxygen-derived free radicals
  • Thermotolerant
  • Yeast

ASJC Scopus subject areas

  • Microbiology

Cite this

Mejía-Barajas, J. A., Montoya-Pérez, R., Salgado-Garciglia, R., Aguilera-Aguirre, L., Cortés-Rojo, C., Mejía-Zepeda, R., ... Saavedra-Molina, A. (2017). Oxidative stress and antioxidant response in a thermotolerant yeast. Brazilian Journal of Microbiology, 48(2), 326-332. https://doi.org/10.1016/j.bjm.2016.11.005

Oxidative stress and antioxidant response in a thermotolerant yeast. / Mejía-Barajas, Jorge A.; Montoya-Pérez, Rocío; Salgado-Garciglia, Rafael; Aguilera-Aguirre, Leopoldo; Cortés-Rojo, Christian; Mejía-Zepeda, Ricardo; Arellano-Plaza, Melchor; Saavedra-Molina, Alfredo.

In: Brazilian Journal of Microbiology, Vol. 48, No. 2, 01.04.2017, p. 326-332.

Research output: Contribution to journalArticle

Mejía-Barajas, JA, Montoya-Pérez, R, Salgado-Garciglia, R, Aguilera-Aguirre, L, Cortés-Rojo, C, Mejía-Zepeda, R, Arellano-Plaza, M & Saavedra-Molina, A 2017, 'Oxidative stress and antioxidant response in a thermotolerant yeast', Brazilian Journal of Microbiology, vol. 48, no. 2, pp. 326-332. https://doi.org/10.1016/j.bjm.2016.11.005
Mejía-Barajas JA, Montoya-Pérez R, Salgado-Garciglia R, Aguilera-Aguirre L, Cortés-Rojo C, Mejía-Zepeda R et al. Oxidative stress and antioxidant response in a thermotolerant yeast. Brazilian Journal of Microbiology. 2017 Apr 1;48(2):326-332. https://doi.org/10.1016/j.bjm.2016.11.005
Mejía-Barajas, Jorge A. ; Montoya-Pérez, Rocío ; Salgado-Garciglia, Rafael ; Aguilera-Aguirre, Leopoldo ; Cortés-Rojo, Christian ; Mejía-Zepeda, Ricardo ; Arellano-Plaza, Melchor ; Saavedra-Molina, Alfredo. / Oxidative stress and antioxidant response in a thermotolerant yeast. In: Brazilian Journal of Microbiology. 2017 ; Vol. 48, No. 2. pp. 326-332.
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