Oxidative stress during the progression of β-amyloid pathology in the neocortex of the Tg2576 mouse model of Alzheimer's disease

Sara Porcellotti, Francesca Fanelli, Anna Fracassi, Sara Sepe, Francesco Cecconi, Cinzia Bernardi, Annamaria Cimini, Maria Paola Cerù, Sandra Moreno

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Alzheimer's disease (AD) is the most common form of dementia, characterized by progressive neurodegeneration. Pathogenetic mechanisms, triggered by β-amyloid (Aβ) accumulation, include oxidative stress, derived from energy homeostasis deregulation and involving mitochondria and peroxisomes. We here addressed the oxidative stress status and the elicited cellular response at the onset and during the progression of Aβ pathology, studying the neocortex of Tg2576 model of AD. Age-dependent changes of oxidative damage markers, antioxidant enzymes, and related transcription factors were analysed in relation to the distribution of Aβ peptide and oligomers, by a combined molecular/morphological approach. Nucleic acid oxidative damage, accompanied by defective antioxidant defences, and decreased PGC1α expression are already detected in 3-month-old Tg2576 neurons. Conversely, PPARα is increased in these cells, with its cytoplasmic localization suggesting nongenomic, anti-inflammatory actions. At 6 months, when intracellular Aβ accumulates, PMP70 is downregulated, indicating impairment of fatty acids peroxisomal translocation and their consequent harmful accumulation. In 9-month-old Tg2576 neocortex, Aβ oligomers and acrolein deposition correlate with GFAP, GPX1, and PMP70 increases, supporting a compensatory response, involving astroglial peroxisomes. At severe pathological stages, when senile plaques disrupt cortical cytoarchitecture, antioxidant capacity is gradually lost. Overall, our data suggest early therapeutic intervention in AD, also targeting peroxisomes.

Original languageEnglish (US)
Article number967203
JournalOxidative medicine and cellular longevity
Volume2015
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Peroxisomes
Oxidative stress
Neocortex
Pathology
Amyloid
Alzheimer Disease
Oxidative Stress
Antioxidants
Oligomers
Acrolein
Peroxisome Proliferator-Activated Receptors
Mitochondria
Deregulation
Amyloid Plaques
Nucleic Acids
Neurons
Dementia
Homeostasis
Anti-Inflammatory Agents
Transcription Factors

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Cell Biology

Cite this

Oxidative stress during the progression of β-amyloid pathology in the neocortex of the Tg2576 mouse model of Alzheimer's disease. / Porcellotti, Sara; Fanelli, Francesca; Fracassi, Anna; Sepe, Sara; Cecconi, Francesco; Bernardi, Cinzia; Cimini, Annamaria; Cerù, Maria Paola; Moreno, Sandra.

In: Oxidative medicine and cellular longevity, Vol. 2015, 967203, 01.01.2015.

Research output: Contribution to journalArticle

Porcellotti, Sara ; Fanelli, Francesca ; Fracassi, Anna ; Sepe, Sara ; Cecconi, Francesco ; Bernardi, Cinzia ; Cimini, Annamaria ; Cerù, Maria Paola ; Moreno, Sandra. / Oxidative stress during the progression of β-amyloid pathology in the neocortex of the Tg2576 mouse model of Alzheimer's disease. In: Oxidative medicine and cellular longevity. 2015 ; Vol. 2015.
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