Oxidative stress effect on progesterone-induced blocking factor (PIBF) binding to PIBF-receptor in lymphocytes

Carlos De La Haba Fonteboa, José R. Palacio, Tamas Palkovics, Júlia Szekeres-Barthó, Antoni Morros, Paz Martínez

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Receptor-ligand binding is an essential interaction for biological function. Oxidative stress can modify receptors and/or membrane lipid dynamics, thus altering cell physiological functions. The aim of this study is to analyze how oxidative stress may alter receptor-ligand binding and lipid domain distribution in the case of progesterone-induced blocking factor/progesterone- induced blocking factor-receptor. For membrane fluidity regionalization analysis of MEC-1 lymphocytes, two-photon microscopy was used in individual living cells. Lymphocytes were also double stained with AlexaFluor647/progesterone- induced blocking factor and Laurdan to evaluate -induced blocking factor/progesterone-induced blocking factor-receptor distribution in the different membrane domains, under oxidative stress. A new procedure has been developed which quantitatively analyzes the regionalization of a membrane receptor among the lipid domains of different fluidity in the plasma membrane. We have been able to establish a new tool which detects and evaluates lipid raft clustering from two-photon microscopy images of individual living cells. We show that binding of progesterone-induced blocking factor to progesterone-induced blocking factor-receptor causes a rigidification of plasma membrane which is related to an increase of lipid raft clustering. However, this clustering is inhibited under oxidative stress conditions. In conclusion, oxidative stress decreases membrane fluidity, impairs receptor-ligand binding and reduces lipid raft clustering.

Original languageEnglish (US)
Pages (from-to)148-157
Number of pages10
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1838
Issue number1 PARTB
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Oxidative stress
Lymphocytes
Progesterone
Oxidative Stress
Cluster Analysis
Fluidity
Lipids
Membranes
Membrane Fluidity
Cell membranes
Ligands
Photons
Microscopy
Microscopic examination
Cells
Cell Membrane
Membrane Lipids
blocking factor

Keywords

  • Laurdan
  • Lipid raft
  • Lymphocyte
  • Membrane fluidity
  • Oxidative stress
  • PIBF-R

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

De La Haba Fonteboa, C., Palacio, J. R., Palkovics, T., Szekeres-Barthó, J., Morros, A., & Martínez, P. (2014). Oxidative stress effect on progesterone-induced blocking factor (PIBF) binding to PIBF-receptor in lymphocytes. Biochimica et Biophysica Acta - Biomembranes, 1838(1 PARTB), 148-157. https://doi.org/10.1016/j.bbamem.2013.08.006

Oxidative stress effect on progesterone-induced blocking factor (PIBF) binding to PIBF-receptor in lymphocytes. / De La Haba Fonteboa, Carlos; Palacio, José R.; Palkovics, Tamas; Szekeres-Barthó, Júlia; Morros, Antoni; Martínez, Paz.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1838, No. 1 PARTB, 2014, p. 148-157.

Research output: Contribution to journalArticle

De La Haba Fonteboa, C, Palacio, JR, Palkovics, T, Szekeres-Barthó, J, Morros, A & Martínez, P 2014, 'Oxidative stress effect on progesterone-induced blocking factor (PIBF) binding to PIBF-receptor in lymphocytes', Biochimica et Biophysica Acta - Biomembranes, vol. 1838, no. 1 PARTB, pp. 148-157. https://doi.org/10.1016/j.bbamem.2013.08.006
De La Haba Fonteboa, Carlos ; Palacio, José R. ; Palkovics, Tamas ; Szekeres-Barthó, Júlia ; Morros, Antoni ; Martínez, Paz. / Oxidative stress effect on progesterone-induced blocking factor (PIBF) binding to PIBF-receptor in lymphocytes. In: Biochimica et Biophysica Acta - Biomembranes. 2014 ; Vol. 1838, No. 1 PARTB. pp. 148-157.
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