Oxidative stress in Nipah virus-infected human small airway epithelial cells

Olivier Escaffre, Hailey Halliday, Viktoriya Borisevich, Antonella Casola, Barry Rockx

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nipah virus (NiV) is a zoonotic emerging pathogen that can cause severe and often fatal respiratory disease in humans. The pathogenesis of NiV infection of the human respiratory tract remains unknown. Reactive oxygen species (ROS) produced by airway epithelial cells in response to viral infections contribute to lung injury by inducing inflammation and oxidative stress; however, the role of ROS in NiV-induced respiratory disease is unknown. To investigate whether NiV induces oxidative stress in human respiratory epithelial cells, we used oxidative stress markers and monitored antioxidant gene expression. We also used ROS scavengers to assess their role in immune response modulation. Oxidative stress was confirmed in infected cells and correlated with the reduction in antioxidant enzyme gene expression. Infected cells treated by ROS scavengers resulted in a significant decrease of the (F2)-8-isoprostane marker, inflammatory responses and virus replication. In conclusion, ROS are induced during NiV infection in human respiratory epithelium and contribute to the inflammatory response. Understanding how oxidative stress contributes to NiV pathogenesis is crucial for therapeutic development.

Original languageEnglish (US)
Pages (from-to)2961-2970
Number of pages10
JournalJournal of General Virology
Volume96
Issue number10
DOIs
StatePublished - Oct 1 2015

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Nipah Virus
Oxidative Stress
Epithelial Cells
Reactive Oxygen Species
Virus Diseases
8-epi-prostaglandin F2alpha
Antioxidants
F2-Isoprostanes
Gene Expression
Respiratory Mucosa
Zoonoses
Lung Injury
Virus Replication
Respiratory System
Inflammation
Enzymes

ASJC Scopus subject areas

  • Virology

Cite this

Oxidative stress in Nipah virus-infected human small airway epithelial cells. / Escaffre, Olivier; Halliday, Hailey; Borisevich, Viktoriya; Casola, Antonella; Rockx, Barry.

In: Journal of General Virology, Vol. 96, No. 10, 01.10.2015, p. 2961-2970.

Research output: Contribution to journalArticle

Escaffre, Olivier ; Halliday, Hailey ; Borisevich, Viktoriya ; Casola, Antonella ; Rockx, Barry. / Oxidative stress in Nipah virus-infected human small airway epithelial cells. In: Journal of General Virology. 2015 ; Vol. 96, No. 10. pp. 2961-2970.
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