TY - JOUR
T1 - Oxidative stress induces p38MAPK-dependent senescence in the feto-maternal interface cells
AU - Jin, Jin
AU - Richardson, Lauren
AU - Sheller-Miller, Samantha
AU - Zhong, Nanbert
AU - Menon, Ramkumar
N1 - Funding Information:
This study was supported by 1R01HD084532 grant ( NIH/NICHD ) to R Menon.
Funding Information:
Samantha Sheller-Miller and Lauren Richardson are predoctoral trainees in the Environmental Toxicology Training Program ( T32ES007254 ), which is supported by the National Institute of Environmental Health Sciences of the National Institutes of Health of the United States and administered through the University of Texas Medical Branch in Galveston, Texas.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/7
Y1 - 2018/7
N2 - Objective: This study tested the mechanism of the oxidative stress (OS)-induced senescence pathway at the feto-maternal interface cells. Methods: Primary amnion mesenchymal cells (AMCs), chorion and decidual cells isolated from the placental membranes of women at normal term (not in labor) were exposed to OS-inducing cigarette smoke extract (CSE) for 48 h. Reactive oxygen species (ROS) was measured using 2′7′-dichlorodihydrofluorescein. Western blot analysis determined phosphorylated (P) p38MAPK and p53 expression. Senescence-associated β-Galactosidase (SA-β-Gal) and matrix metallopeptidase 9 (MMP9) histochemistry were used to measure senescence and inflammation respectively. Cotreatment of cells with the antioxidant, N-acetyl cysteine (NAC), or the p38MAPK inhibitor, SB203580 (SB), verified the activation specificity. Results: CSE increased ROS production from AMCs, chorion cells, and decidual cells (P < 0.05) compared to controls. Western blot analysis determined that CSE induced p38MAPK activation (P < 0.05) and cotreatment with NAC inhibited ROS production and p38MAPK activation (P < 0.05) in all cell types. CSE did not increase p53 phosphorylation in any of the cells; however, AMCs showed constitutive P-p53 expression. CSE increased senescence in AMCs and chorion cells compared to controls (P = 0.01 and P = 0.003, respectively); however, senescence was not observed in decidual cells. Senescence was significantly reduced following cotreatment with SB and NAC (AMCs; P = 0.01 and chorion; P = 0.009). CSE increased MMP9 in all cells that was reduced by NAC. Conclusion: OS induced p38MAPK activation and inflammation in all cell types that was associated with senescence in fetal cells but not in maternal cells.
AB - Objective: This study tested the mechanism of the oxidative stress (OS)-induced senescence pathway at the feto-maternal interface cells. Methods: Primary amnion mesenchymal cells (AMCs), chorion and decidual cells isolated from the placental membranes of women at normal term (not in labor) were exposed to OS-inducing cigarette smoke extract (CSE) for 48 h. Reactive oxygen species (ROS) was measured using 2′7′-dichlorodihydrofluorescein. Western blot analysis determined phosphorylated (P) p38MAPK and p53 expression. Senescence-associated β-Galactosidase (SA-β-Gal) and matrix metallopeptidase 9 (MMP9) histochemistry were used to measure senescence and inflammation respectively. Cotreatment of cells with the antioxidant, N-acetyl cysteine (NAC), or the p38MAPK inhibitor, SB203580 (SB), verified the activation specificity. Results: CSE increased ROS production from AMCs, chorion cells, and decidual cells (P < 0.05) compared to controls. Western blot analysis determined that CSE induced p38MAPK activation (P < 0.05) and cotreatment with NAC inhibited ROS production and p38MAPK activation (P < 0.05) in all cell types. CSE did not increase p53 phosphorylation in any of the cells; however, AMCs showed constitutive P-p53 expression. CSE increased senescence in AMCs and chorion cells compared to controls (P = 0.01 and P = 0.003, respectively); however, senescence was not observed in decidual cells. Senescence was significantly reduced following cotreatment with SB and NAC (AMCs; P = 0.01 and chorion; P = 0.009). CSE increased MMP9 in all cells that was reduced by NAC. Conclusion: OS induced p38MAPK activation and inflammation in all cell types that was associated with senescence in fetal cells but not in maternal cells.
KW - Amnion mesenchymal cells
KW - Chorion
KW - Decidua
KW - Oxidative stress
KW - Senescence
KW - p38MAPK
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U2 - 10.1016/j.placenta.2018.05.008
DO - 10.1016/j.placenta.2018.05.008
M3 - Article
C2 - 29941169
AN - SCOPUS:85047239297
SN - 0143-4004
VL - 67
SP - 15
EP - 23
JO - Placenta
JF - Placenta
ER -