@article{f0060a3fdf6e4e0aa6e85c9dde906818,
title = "Oxidized glutathione levels in erythrocytes of glucose-6-phosphate-dehydrogenase-deficient subjects.",
author = "Srivastava, \{S. K.\} and E. Beutler",
note = "Funding Information: per ml. blood) and centrifuged at 1000 g for 30 minutes. The plasma and buffy coat were aspirated and the R.B.c. were washed once with isotonic phosphate-saline solution. G.S.H. was estimated by the 5-51-dithio(bis)-nitrobenzoic-acid method (Beutler et al. 1963). G.S.S.G. was estimated on trichloroacetic- acid filtrates of N-ethyl-maleimide-treated R.B.c. with gluta-thione reductase and N.A.D.P.2H (Srivastava and Beutler 1968), Results and Discussion R.B.c. G.S.S.G. levels were estimated in 32 normal and 6 G.-6-P.D.-deficient subjects. 3 of the G.-6-P.D. deficient subjects could be classified as having G.-6-P.D. A-, and 2 as having the Mediterranean mutant. 1 subject had non- spherocytic congenital haemolytic disease (G.-6-P.D. Torrance) Tanaka and Beutler 1968). In most cases G.S.H. determinations were also carried out. As shown in the accompanying figure, G.S.S.G. levels of G.-6-P.D.- deficient R.B.C. were substantially increased. The average G.S.S.G. content of normal R.B.C. was 3-6 (s.D. 1-4) m\µmoles per ml.; that of G.-6-P.D.-dencient R.B.C. was 10-28 (s.D. 3-0) m\µmoles per ml. Because, as has previously been shown (Beutler et al. 1955), the average G.S.H. level of G.-6-P.D.-deficient R.B.C. is diminished, the G.S.S.G./G.S.H. ratio was strikingly raised. As before, the total glutathione content of G.-6-P.D.-deficient R.B.C. was found to be diminished, but this is now readily explained by the out- ward transport of G.S.S.G. from R.B.c. (Srivastava and Beutler 1967, 1968). These findings are in concordance with the lowered N.A.D.P.-reducing capacity of G.-6-P.D.-deficient R.B.c. We believe that they fill a gap in our understanding of the overall metabolic derangement of G.-6-P.D.-deficient R.B.C. This work was supported, in part, by Public Health Service grant no. HE 07449 from the National Heart Institute, National Institutes of Health, and Public Health Service grant no. HD 01974 from the National Institute of Child Health and Human Development. Requests for reprints should be addressed to E. B.",
year = "1968",
month = jul,
day = "6",
language = "English (US)",
volume = "2",
pages = "23--24",
journal = "Lancet",
issn = "0140-6736",
publisher = "Elsevier B.V.",
number = "7558",
}