Oxygen free radical content and neutrophil infiltration are important determinants in mucosal injury after rat small bowel transplantation

Luca Cicalese, Paolo Caraceni, Michael A. Nalesnik, Andre B. Borle, Wolfgang H. Schraut

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Mucosal injury is an immediate event following revascularization of small intestinal grafts in the context of transplantation (SBTx). The generation of oxygen free radicals (OFR) and tissue infiltration by activated neutrophils are consequences of ischemia and reperfusion and known causative factors of tissue injury; to delineate their role in the reperfusion injury occurring after cold preservation of the intestine and subsequent transplantation was the aim of this study. Prior to orthotopic SBTx in Sprague-Dawley rats, grafts were stored in cold (4°C) Ringer's lactate solution for 1 (n=6), 2 (n=7), and 4 hr (n=7). Small bowel biopsy specimens were obtained before harvesting, at the end of the (cold) ischemic period and immediately before unclamping (i.e., before revascularization) and 30, 60, 120 min, and 24 hr after transplantation to evaluate tissue injury by histology, OFR production, (measured by luminol-enhanced chemiluminescence [LCL]), and the degree of neutrophil infiltration by myeloperoxidase staining. Reperfusion of the graft significantly worsened the histologically graded mucosal injury compared with that seen before unclamping. However, 24 hr after engraftment, mucosal morphology was restored almost completely. OFR production increased significantly during the early phases of reperfusion (30, 60, and 120 min) and returned to control values after 24 hr. Reperfusion of the graft was associated with a marked increase in the number of mucosal neutrophils. The present study indicates that OFR production and neutrophilic infiltration commence and progressively increase with graft reperfusion. These changes parallel the mucosal injury. Ischemic intervals of 4 hr were not associated with a statistically significant greater ischemic-injury patterns compared with 1- and 2-hr intervals. The profound changes associated with reperfusion probably overshadow the minor, yet likely, progressive injury patterns associated with longer ischemia times.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalTransplantation
Volume62
Issue number2
DOIs
StatePublished - Jul 27 1996
Externally publishedYes

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Neutrophil Infiltration
Free Radicals
Reactive Oxygen Species
Reperfusion
Transplantation
Wounds and Injuries
Transplants
Ischemia
Luminol
Thromboplastin
Luminescence
Reperfusion Injury
Peroxidase
Intestines
Sprague Dawley Rats
Histology
Neutrophils
Staining and Labeling
Biopsy

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Oxygen free radical content and neutrophil infiltration are important determinants in mucosal injury after rat small bowel transplantation. / Cicalese, Luca; Caraceni, Paolo; Nalesnik, Michael A.; Borle, Andre B.; Schraut, Wolfgang H.

In: Transplantation, Vol. 62, No. 2, 27.07.1996, p. 161-166.

Research output: Contribution to journalArticle

Cicalese, Luca ; Caraceni, Paolo ; Nalesnik, Michael A. ; Borle, Andre B. ; Schraut, Wolfgang H. / Oxygen free radical content and neutrophil infiltration are important determinants in mucosal injury after rat small bowel transplantation. In: Transplantation. 1996 ; Vol. 62, No. 2. pp. 161-166.
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